XB-CLONE-3944005
Clone Name: xthr21N04
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Gene:
col1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN103910 [+]
acttaccttcctgcgccttatggccactgaagcctctcagaacatcacttaccattgcaagaacagcgttgcttacatggaccaagctacaggcaacctg
aagaaggccctgctcctgcaaggctccaatgaaatcgagatcagagcagagggcaacagccgattcacatacagtgttgtagaggatggttgcacgcaac
acacaggagaatggggcaagacagtaattgattacaagacaacaaagacatcacgcctgcccattaccgatgtggcacccatggatatcggagccccaga
tcaggaattcggaattgacatcggaccagtctgcttcgtgtaaaacaaaacacacaacacaaataccaggacaagagattatctcatgtggacttgaaat
tgttgttcctctcatctctaaaacttttcttgtttccgtaagcattacacgaaaaagcgaaccgatagtcatgctttgaagaacatctctcaaaggacag
accacgcccatgtgtgtgtgtgtgtatgtgtttgtgttcacgggagggagagttccaccaatctgatcagccacgccccttgcatacatgaattggcctt
cctaggaccttgaagacagaatgggacaagcccaaaaagaagaatgggatatacttaaacatactgttcctggatttgaaatgact
3' EST: CN103909 [+]aaggtgcattacatgaaatgttttggtttactcctcttcttctctggggaacctggctggggtcattgatcccacattctagtcatttcaaatccaggaa
cagtatgtttaagtatatcccattcttctttttgggcttgtcccattctgtcttcaaggtcctaggaaggccaattcatgtatgcaaggggcgtggctga
tcagattggtggaactctccctcccgtgaacacaaacacatacacacacacacacatgggcgtggtctgtcctttgagagatgttcttcaaagcatgact
atcggttcgctttttcgtgtaatgcttacggaaacaagaaaagttttagagatgagaggaacaacaatttcaagtccacatgagataatctcttgtcctg
gtatttgtgttgtgtgttttgttttacacgaagcagactggtccgatgtcaattccgaattcctgatctggggctccgatatccatgggtgccacatcgg
taatgggcaggcgtgatgtctttgttgtcttgtaatcaattactgtcttgccccattctcctgtgtgttgcgtgcaaccatcctctacaacactgtatgt
gaatcggctgttgccctctgctctgatctcgatttcattggagccttgca
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: