XB-CLONE-3920864
Clone Name: xthr09P16
|
Gene:
ocm4.5
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CN119265 [+]
gagaagtgttaatactcaaaaggttgctcagagccctttgctcctgttaaagaggaattcagatttaccatggcattcggtggaatcctgagtgaggctg
acatctctgctgccctgcagaactgccaagcccctgactccttcaacttcaaaactttctttgcccagcctggcctgagcagcaggtctgcagatgatgt
gaagaatgtctttggcatcctggaccaggacaggagtggcttcattgaggaagatgaactgaaactgttcctccagaacttcagcgcaggtgccagggct
ttaactgatgctgagaccaaggccttcctggcagctggagactctgatggtgatggcaaaattggagttgaagaattccaggctctagtcagaccttgaa
aatgaagaccacagacttccatgaaagatgaagttgcaggaattttggcttttcttagttaaattgaaagaaatggaacccggttgggcagaatgcagaa
acctatggacaagcaaaggaagggctttgtgctgcatagaagaaacgttgtcgggaaaccagcaatttatctaagagttgcctaattaacattatttatt
taacatgttatataccactggtctctgatcatatcagcatgacttttgtaaattagcatgtgattcttctttcagac
3' EST: CN119264 [+]ttttttatggtgctgcctcagtctgaaagaagaatcacatgctaatttacaaaagtcatgctgatatgatcagagaccagtggtatataacatgttaaat
aaataatgttaattaggcaactcttagataaattgctggtttcccgacaacgtttcttctatgcagcacaaagcccttcctttgcttgtccataggtttc
tgcattctgcccaaccgggttccatttctttcaatttaactaagaaaagccaaaattcctgcaacttcatctttcatggaagtctgtggtcttcattttc
aaggtctgactagagcctggaattcttcaactccaattttgccatcaccatcagagtctccagctgccaggaaggccttggtctcagcatcagttaaagc
cctggcacctgcgctgaagttctggaggaacagtttcagttcatcttcctcaatgaagccactcctgtcctggtccaggatgccaaagacattcttcaca
tcatctgcagacctgctgctcaggccaggctgggcaaagaaagttttgaagttgaaggagtcaggggcttggcagttctgcagggcagcagagatgtcag
cctcactcaggattccaccgaatgccatggtaaatctgaattcctctttaacaggagcaaagggctctgagcaaccttttgagtattaacacttctcc
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: