XB-CLONE-3919662
Clone Name: xthr02J06
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Gene:
ube2i
,
spcs2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN119867 [+]
gagggggctttgtcccgagacaaagagcaggaatcaccgccgcccgagccgcattctctgggggtgaagccgccgccgcaggaaccctgaaatgtctggc
atagccctgagcagacttgcacaggagagaaaagcgtggagaaaagaccatccttttggttttgtggcagtaccaacgaaaaatccagatggcacaatga
atttgatgaactgggaatgtgctattccaggcaagaaagggaccccttgggaaggtggcttatttaaattacggatgctttttaaggatgattatccctc
ctcacctcctaaatgtaaatttgagccacccctttttcacccgaatgtctatccttcaggcacagtgtgtctgtctatcttagaagaagataaggattgg
aggccagcaatcacaattaaacagatcttgttaggaatacaagaacttctaaatgaaccaaatatacaagatccagctcaagcagaggcatacacaattt
actgccaaaacagagttgaatatgaaaaaagagtcagagcacaagc
5' EST: CN107217 [+]gcccattatggccggggggaacccccaggggtgatacagtaaggattgtgcgttctttccctgcctctggggggagcttgtcgtgctttgctcttcttaa
cgtcctcagtgggaggagccacaggcgccttgggcagcggaagggcgggtactaaggttttgtcctgaaatactcactgctcccacttattaaacgtagt
tatttcatttcccgcatttagtagagggaattgtcatagagaaatgtgcagaaaggagtccgtgcccgactctccgaccatttaggctgaagacacacgg
agctacttagtagcagctacagaaacagacaatgctgatcatttactgataattgtctctgtgtgtttagcaggggcaattctcagtattgtctatggca
ggggattttctggcgtttagtagctgctacaaagtagctctgtgtgtcttcacccttatgccgagcattagcatcaacggcaacagccctgattggctac
aaaataccatcatggctggggatt
3' EST: CN107216 [+]agaaaacggcagatcagaaagacgtgctcagcaggggtttgtggggggcggaatgtgacaaaagggggacaaaaccaaaaacagtaacagatagaaaaat
gctcctttttttattggccgaaagagagcgctgagctgttacatacgtgtcgggtcctttccgtttctcagggaacagaaacaaactgaaacgacacgag
gttttattccaccaaataaatatcaaaaaaaaatataatggacagatttctgcaaaactaaggttttgtcctgagatccataaaaatgtacattttagtt
tgagggggggggatttagtgacacaaggatgggtaccctgcctctgtgccgaccaatccccagccatgatggtattttgtaacccatcagggctgt
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: