XB-CLONE-3919636
Sequence:
5' EST: CN119880 [+]
gctcttcctcgggctgtctgcggcgaaggcggacatctcgaaccggtaccatggcggccctaagacctctcaccaagcccaagatagtgaagaagcggac
aaaaaagttcatccgccaccagtcagatcgctatgttaagattaagcgtaactggcgcaagcccagaggtattgataacagagcccgcagaaggtttaag
ggacagatcctgatgcccaacatcggttatggtagcaacaaaaagacaaaacacatgttgcccacaggatttaagaagtttctggtgcacaatgttaaag
aattggaggttcttttgatgagcaacaagactttctgcgcagaaattgcccacaatgtctcctctaaaaaccgtaagaccattgtggaaagagcagcaca
gcttgccatcaaagtgacaaaccccaacgccagacttcgcagcgaggaaaacgaataaacttcctgtctctgatgttgctctttgtaataaatgaaaaag
taaaggaaaaaaa
5' EST: CN107246 [+]gctttctctgcccctgtccaccatgcctaaagggaagaaggccaaggggaagaaggtggcacccgccccttccgtcgtgaagaaggccgaggccaagaag
gtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctgacccgctttgtcaaatggccccgtt
acatcagactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcacccaagccttggaccgtcagacagccac
acagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggctgagcagaaggccgctggcaaggga
gacgttcccaccaagagaccccctgtactcagagctggtgtcaacactgtcaccacccttgtggaaaacaagaaggctcagcttgttgtgatcgcccatg
atgtcgatcccatcgagctcgtcgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtcaagggcaaggccagactgggccgcct
cgtgcacaggaagacctgcaccagcgtagcactcacacaggttaaccctg
3' EST: CN107245 [+]tgcatgtcttcctgtgcacgaggcggcccagtctggccttgcccttgacaatgcaatatgggactcccatcttgcggcacaaagcagggaggaacactac
gagctcgatgggatcgacatcatgggcgatcacaacaagctgagccttcttgttttccacaagggtggtgacagtgttgacaccagctctgagtacaggg
ggtctcttggtgggaacgtctcccttgccagcggccttctgctcagccctggccagcagccttttcttcttttcctgtttattctcaagtctgtacttgt
gggccagtttgaacagctgtgtggctgtctgacggtcccaaggcttgggtgaactggttgattgcatgtggga
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: