XB-CLONE-3918628
Clone Name: xthr04M18
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Gene:
eif1ax
,
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120384 [+]
ggtgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaaaccagccccacca
aaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaagggtaaaaagggagcaaagg
gcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaaaagtgacgaggcccctgcttctgatggagg
cgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcccttgtacaattcagaggaatatttttatca
actattttgtaaatgcaagtttttttagtagtttaattctagaaaacacatttttaaaataggagggaatccccaccatttttttgttgtaaaaaaaatt
ttttttaaacacttatgatttggtacattgcccctgtatggtttgtaaaggctatgaatactataccagttaatactacaccac
3' EST: CN117757 [+]cctcatgtatatactttcacaatttaatagttacttcccagaacagtatgaaacaactgaagtatcagaagtgcagaagtacagaagtaaaacaatcaca
aagaccaggatgctatatctcaaatgtataaacttgggggttacgtaaaatcatgaatgcggaagtttcagacacagcaaagtaatcatggataataaat
gtgcagtgaagagataccgcaattccttaatctaatgagtgtccaagcaaattaaatcatgataaatagagggacttaaaaaccatggtgcagaccagat
ggagaaatgaacacaaggcagcatgtccccatcttttatgggcagagcacaatagagtagctcaggttaggaaattaaaatttcactgcttagattttgc
agatcaagcattgttgacctctacactcaatgaatataaatacattaaaactgaaaataaattttattcagtggagatcatcatctctttaggagggctg
cactcaaaatcccaaacaaaaccttgtcgaacaatccttggaatttggaatggaataatcgtctcacattagatatcatcaatatcttcatcgtcatctc
caatgtcatcaaactgaatttcatcatcatcaccaggaccaaatgtgtctgtttcattaattttagcatgttctggaagctctccataggctttcagact
tctggcttcatctgcattgtattttagaataacatctgctttgttgtcttgatagtctcgtaggccaaccaatatgatgtctgatgt
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: