XB-CLONE-3918188
Clone Name: xthr05E23
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Gene:
twist1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120604
[+]
gagatacagtgactggggatcctcaagtgccagaattggttcatttagaagaattcatcacatgttttaaacaacataaaaacattggaatgcaaatatg
cttgagttttaataaaaaagacgaataacactaaaa
5' EST: CN118100
[+]ggagttagagagacgttggcgcctggggacagactgtgctatagggctgggcgcactccccccacccacccacccacctcagcaccttggagaagacttt
gctcgggagatctgttgggacagagcctgccctctgcccgggaccttctcctgattgtgctgggagaaaatgatgcaggaagagtccagctccccagtct
ccccagtagacagtctaagtaacagcgaggaagagcttgacaggcagcacagcaagaggggatgcagaaagaagcgatccactaggaaaagccctgagga
tcccgacagccccatctcagtgaagcgcaacaagaagtccagcagcaccggcagcagcccgcaatcctttgaagaactgcaaagccagagggtcatggcc
aatgtcagggagcgccagaggacccagtccctaaacgaagccttcgcagccttgaggaagatcatccccaccctcccctctgacaaactgagtaaaatcc
agactctcaaactggcatccagatacatcgactttctctgccaggtcttacagagcgacgagctggactccaaaatggccagctgcagttatgtggccca
tgagaggctcagctatgcattttcagtgtggaggatggagggagcatggtccatgtctgcatctcactaaca
3' EST: CN118099
[+]tacaatgatatttacaaaacaaccataaaaaatatgaatgcatttagacaccggatctagttgcatttttttttttaaacatggatcaataaataaataa
atgttaactttttttctcaggtgatgaataaagagatgccgccgcttgtatagaaacagttttctgaaccaagaatcaaatagcaaaaatagtatttttt
tccttcctatttaaaaaaatggatttcgatagaaaccttcaagaatgggaatgttcaaatattttcctctaaagtggcttcaaaggcacgactggtggta
acgcatgcatgcagtatacaaaaagtacaaggaaaaacgaattaaagaaaataatttccaacattcttcgtcaaagtccagatgagagattgttcaggat
cagaatggaatggatttggcgaacctacacggctccctgctgtataaattcagtgtcccagggcagatttgctctgtaagtgctccgcacgccttcattc
gcttcggccctttcaccctaatgcacaaaaatcctcagatttaattgttttgggtatccagctccagtttctacactgtccatttcccattcattggtta
aagtatcgtctttctgtgtggcctgtgctgtagtggcattcctgttagtgagatgcagacatggaccatgctccctccatcctccacactgaaaatgcat
agctgagcctctcatgggccacataactgcagctggccattttggagtccagctcgtcgctctgt
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: