XB-CLONE-3917880
Clone Name: xthr05D03
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Gene:
actg1
,
rps23
|
Species: Xenopus tropicalis
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|
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Sequence:
5' EST: CN120758 [+]
gtctcccggtgggttgtgggattcgctctttccgcagtcggccttgacacagtcaagatgggcaagtgccgtggtcttcgtacagcaagaaagctccgca
accaccgccgtgagcaaaaatggcatgataagcagtacaagaaggcccatctgggaaccgctctgaaggccaacccatttggaggtgcttctcatgctaa
aggaattgtcctggaaaaagtcggtgtagaggctaagcagccaaactctgctatcaggaagtgcgtcagagtgcagctgatcaaaaacggcaagaaaatc
actgcattcgtaccaaacgatggttgtctgaattttattgaggaaaacgacgaggttctggtggcaggatttggacgtgctggtcatgccgttggtgata
ttcctggtgtccgattcaaggtggttaaagtagccaatgtctccctgctggccttgtacaaaggaaagaaggagagaccaagatcatagacatggattga
aaagacatttaa
5' EST: CN118393 [+]gctgacagaatgcagaaggaaatcactgccttggcccccagcaccatgaaaattaagatcattgccccacctgaacgcaaatactctgtctggattggtg
gctctatcctggcctccctctccactttccagcagatgtggatcagcaagcaggagtatgatgaatctggcccatccattgtccaccgcaaatgcttcta
aatggactgctagcagatgcgttgcatttgctgcgtgagttcttcaaagtattaatttgccttggcaaatgtgtacgcctcatgctagcctcttgaaact
ggaaaaagggccaacttccaaatgaacttatcctaggaattggagacacacatagtcactgttgggggattttgtaatgcaagacttgtgcttgtctctt
caaatcattcctgtggcatcaatgctagcatattgtaccctgtacacatctcttatattgcctggttggcatacagtctgggtgacacttaagctaagct
ttacgtagaaagaagcatttctgtgaaaggctgccgatctgcgacagtattgcgaatgacacttctcctctaggttacaggggctgtttatggagaagtc
gagttttggagatgtgtacagggtatctgaagtttagacttccacac
3' EST: CN118392 [+]ggtgttttggaagttcaattctcatctggatgactacaaattttggttttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaatta
acttggatgcataaatttacaggtgttaactgcaacatttgtaactttcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagact
ggttcttgggcctgagtgtcaatttccaaccttactataaactggaaagatagaagagattccttcatgaactgaccagcctccgacatgaaacaatgga
gtattaagcgcttaactgtgtgtggaagtctaaacctcagataccctgtacacatctccaaaactcgacttctccataaacagcccctgtaacctagagg
agaagtgtcattcgcaatactgtcgcagatcggcagcctttcacagaaatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgcca
accaggcaatataagagatgtgtacagggtacaatatgctagcattgatgccacaggaatgatttgaagagacaagcacaagtcttgcattacaaaatcc
cccaacagtgactatgtgtgtctccaattcctaggataagttcatttggaagttggccctttttccagtttcaagaggctagcatgaggcgtacacattt
gccaaggcaaattaatactttg
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: