XB-CLONE-3917858
Clone Name: xthr05F05
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Gene:
ube2n
,
pdcd5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120769 [+]
ggggacttacaatgcagctggtgtgcaccttcctgctcctctgtgctctcactatacagaaaggcagggccttacagtgttacacgtgtgtgggctccag
tgaccaggactgcaaccgccaaggaactcagcaatgcccccaagagtcagacgcctgtgccatcatgcgcggccagtctagcggagtcatgaagtcctgc
tcctataagtcattctgcgaccgagccatgcgcgacggtaacagggcgcccggaacgcgcgtccaatgctgcttctccaacaactgtaacagtgacagca
aagggtccggcagccaactcagcgccagtggtgtccgcctcctactctctgtggcgttgggtttctgcctcctattcatcagctgaggactgggtcggat
tggcctaccagagtaccagacgatctccaggttgaccccagccaaggacaattcctatcagcccttccttatttccaccctaggtctatagctaactgtc
atgagagtgggccctcagtgtcaggttccctggtatgccctaggtcgcccagtctgtggagtctcccaacatactgtcctcttctgctactggacttaca
tgatcc
5' EST: CN118413 [+]gctcacttgtgcgtgacagggggatccggtggcacgcagagagttggaggctgcacgcattataagacaggatggccgggctgcctcgcaggataatcaa
ggaaacccagcgtttactggcagagccagtccccggaattaaggctgagccagatgaaaacaatgctcgctatttccatgtgcttatcgctggtccacag
gattccccctttgagggagggacatttaaacttgaattatttcttccggaagaatacccaatggcagcgcctaaagtacgtttcatgaccaaaatctatc
accctaatgtagacaagttgggaagaatatgtctagatattttgaaagataaatggtcaccagctctgcaaatccgtaccgtgctcctatcaattcaggc
tttgttaagtgctcccaacccagatgacccattagccaatgatgtagctgagcagtggaagacaaatgaagcccaagccatagaaacagccagagcatgg
actaggctacatgctatgaataatatttaaattcggcttgatcttcaagtgcacatcatttctcctgttctgccaagacctcttccctttatttttgttt
tgtttttgtttgtgtttaatggacacagtc
3' EST: CN118412 [+]tctgaagtagctggctaagagcatatacacaaagcgcaggttagctgctaccaaacacattaaagacaataataaaaagggtaaattcaatgcaaacatg
ttaggcttcattaacttggtacaatgctgacatttccttagaaatttgatgaatgtaaatatacagtgtataaaacaaaaaatgttatgatataagcatt
ctctgcttcatggatggatttttagaatcatctctaattctctgtttaataaatcgttcctacaggggaagggaggatggggtgcaagtacatgttactt
tgaatacagatttgaccaatgcaaaaacaaaagtgcatctaaaaagaaagggaggggcaatgacacattgttttaaagttaacagagccaaacaaatagt
tgttttatcttaaaaattaaaaaaaaaaaccaaactgtttcagcaactaacctaactctaccttcattcacagtgctcatactcaaatcatgatgggtat
aatggacatcaagaacttttgccacccctttgaacattgtggaacatgctgacacgatggattacagcctctgcgcatgcgatacaacagtgaattagaa
caagacatattatttgctaatgtgcatttaatcaccaaagatgaccatgtctgggctttttattccatatattttattaaaactgtgtccattaaaca
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: