XB-CLONE-3917697
Clone Name: xthr05D16
|
Gene:
hmgn1
,
tuba1cl.3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CN120850 [+]
ggaactagttctctggctcgcatattaaccgcttgcaagcgctagggagccgactagtctttgtgagtactccatctgcaggagggctcgcacatagaaa
aaacaaatatatataaataagtgcaagcaagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctt
tggattcaagcggtgcacacaagccctcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagagg
agatcggcacggttgtcggctaaaccagccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggaga
aaaaagttccagcaaagagtaaaaagggagcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggaga
aacaaaaagtgacgaggcccctgcttctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgt
acc
5' EST: CN118550 [+]gagaactccaatatccgagcacataacttatcggaaccaggagtctatcagccaacaacatgagggagtgcatctcagtccacgtggggcaggcaggagt
gcagattggcaatgcatgctgggagctgtactgcctggaacatgggatccaaccggacggccagatgcccagcgacaaaaccattgggggaggggacgat
tccttcaacaccttcttcagtgcgaccggagccgggaagcacgtcccccgcgctgtgtttgtggacctggagcccactgtgattgatgaggttcggaccg
gtacctaccggcagcttttccaccctgagcaactcatcacaggcaaggaagatgccgccaataactacgcccgcggccattacaccatcggcaaggagat
cattgacctggtgctggacaggatccgcaagctggcggatcagtgcacaggactccagggcttcctcatcttccacagcttcggggggggcactggctct
ggattcacctccctgctgatggaacgtctctctgttgactatggcaagaagtccaagctggaattctccatctatccagctcctcaaatctccacggctg
tggtggaaccctaac
3' EST: CN118549 [+]cttcttctccctctccctccacgctgtcagttcctatctcctcataatccttctccagggcggccatatcctcgcgggcctcagagaactccccttcctc
cattccctcccctacataccagtgcacaaaggcacgcttggcatacataaggtcaaacttgtgatccaggcgagcccaggcctcagcaatggcggtggtg
ttgctcaacatgcacacagctcgctgtaccttggccagatccccacccggaaccacagttgggggctggtagttgataccaaccttgaaaccagttgggc
accagtccacaaactggatggtgcgcttggtcttaatggtggcaatagcagcattgacatctttgggcaccacatcacctcggtacaacatacaacaagc
catgtatttaccatgacgggggtcacatttcaccatctgattggctggctcaaagcaagcattggtgatctcggatacagagagttgctcatggtaagct
ttctctgcagagataacaggggcataggtggccagagggaaatggatacgggggtagggtaccaagttggtttggaattctgtcaggtccacattcaggg
ctccatcaaatctgagggaggctgtgatagaggacacaatctggccaatcaggcggttcaggttggtgtaggttggg
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: