XB-CLONE-3917659
Clone Name: xthr05H06
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Gene:
rpl7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120869 [+]
gtttcatgtggagaaatgtgctgtgtcattcgcaaagtgcatgaatggtcagcggcgggttattgcatcactcgaaccgggaattagggatttcctaaag
ctgtcccgtaaagtgttggatggattattgcggtgcgcaccggtgcagatttgtgtacagaggggactatgatgctgactgggaccgtagctgtagccga
caagagccctgaacttgcaccactgaagaaatcggctgatctaaacgccagtaatgatggtgggcatcggaaatttacttctaaccaaagacaactgatt
tgtgagagaatctcccagtggtgtacaagaagtaaaatccttattgcagcagctattgtagtagttattgcagcattgggccgtggaatatgtattggca
gaagcttagttcaacagcaggtacccaccctgggtgtctgcaattcttggcaaactcagaattttcctactcggcaatttgcctgtctgcctcttgtcta
cgctgtctgtattttcacatgcactctctctgctgtgaggaaagggaccgtgccctcctgcctgcccggacaagtggaccttgtatattgggacctgcta
ttttgcatcccatgaaactcaagatataattattc
5' EST: CN118585 [+]gctttttctctgcccctgtccaccatgcctaaagggaagaaggccaaggggaagaaggtggcacccgccccttccgtcgtgaagaaggccgaggccaaga
aggtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctgacccgctttgtcaaatggccccg
ttacatcagactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcacccaagccttggaccgtcagacagcc
acacagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggctgagcagaaggccgctggcaagg
gagacgttcccaccaagagaccacctgtactcagagctggtgtcaacactgtcaccacccttgtggaaaacaagaaggctcagcttgttgtgatcgccca
tgatgtcgatcccatcgagctcgccgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtcaagggcaaggccagactgggccgc
ctcgtgcacaggaagacctgcaccagcgtagcactcacacaggttaaccctgaggacaaaggtgcc
3' EST: CN118584 [+]aagatcggtacatttaaccaagcttggtggccagctcctttgcttttgccttctccagtttggcgatgcgggcaatggacttggggcccaggacaccacc
tccccagtgacgacggatctcatcgaatctgtcattgtagttggtcttaactgcttccaccaacttggcgagggcacctttgtcctcagggttaacctgt
gtgagtgctacgctggtgcaggtcttcctgtgcacgaggcggcccagtctggccttgcccttgacaatgcagtatgggactcccatcttgcggcacaaag
cagggaggaacacggcgagctcgatgggatcgacatcatgggcgatcacaacaagctgagccttcttgttttccacaagggtggtgacagtgttgacacc
agctctgagtacaggtggtctcttggtgggaacgtctcccttgccagcggccttctgctcagccctggccagcagccttttcttcttttcctgtttagtc
tcaggtctgtacttgtgggcca
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: