XB-CLONE-3917587
Clone Name: xthr05N16
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Gene:
nme2
,
rpl28
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Species: Xenopus tropicalis
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Sequence:
5' EST: CN120905 [+]
gcttctttggtttcctgccccccggagagtgaaagatggctgccaacaaggaacgcacttttattgccatcaagccagatggagtccagcgcggcctcat
gggggaaatcatcaaacggtttgagcagaggggtttttacttggttgccatgaagtttgtgcaggcttctaaagacttgctcaagcagcactacatcgac
ctgaaggatcggccattttaccccggactggttgattacatgagctccggccccgttcttgccatggtctgggagggactgaatgtggtaaaaactggcc
gagtgatgcttggcgagaccaaccctgccgactccaagcctggcaccatccggggagacttctgcatacaggttggcaggaacatcattcacggcagcga
ctcagtggaaagtgccaataaggaaatcgccctgtggttcgaagacaaggagctggtggagtacaaatgctgcgcgcacgagtgggtgtacgagaactga
gcccccttctgccttccgttacctctttactccaataaagtgttaccatcctgccccaa
5' EST: CN118655 [+]gttcctctagcagcgcggagagaggtgcctgaaaatgtctgcccacctgcaatggatggttattcgcaattgctccagctttcttgtcaaaagaaaccat
cgcatctttagtacagagccaaacaatctaaaagcaagaaactcatttcggtacaatggcctcatccacaagaaaacagtcggtgttgagcctgcagctg
atggaaagggggtcattgttgttctaaagaaacgtgcaggtcagcgcaaacctgcaacatcttatgaaaaaattaccatcaacaagaactcccgctccac
tctgagcagcgtgcgtcacattatccgcaagaacaactacaggaaggatctccgcatggctgctctccgccgtgctagcgccatcctgaagagccagaaa
ccagttgtggtaaagaagaagcgcacccgggctgccaagactgcataaatgtgttctgcagatgtgtgcaataaaatggtttcatatgaaaaaaaaaaaa
aaaaaaaa
3' EST: CN118654 [+]ttatgcagtcttggcagcccgggtgcgcttcttctttaccacaactggtttctggctcttcaggatggcgctagcacggcggagagcagccatgcggaga
tccttcctgtagttgttcttgcggataatgtgacgcacgctgctcagagtggagcgggagttcttgttgatggtaattttttcataagatgttgcaggtt
tgcgctgacctgcacgtttctttagaacaacaatgaccccctttccatcagctgcaggctcaacaccgactgttttcttgtggatgaggccattgtaccg
aaatgagtttcttgcttttagattgtttggctctgtactaaagatgcgatggtttcttttgacaagaaagctggagcaattgcgaataaccatccattgc
aggtgggcagacattttcaggcacctctctccgcgctgctagaggaacc
Library:
Description: Xenopus tropicalis polyA+ RNA was obtained from pool of heads and retinas from tailbud stages 25-35 cDNAs were synthesized using the SMART system of CLONTECH and directionally cloned into pCMVSPORT6_xthr, a modified version of pCMVSPORT6 allowing directional cloning using asymetric SfiI sites. For antisense RNA synthesis, use T7 promoter and for sense RNA use SP6 promoter. Library constructed by Drs. N. Pollet, M. Perron, M. Wegnez, A. Mazabraud (CNRS UMR 8080, Universite Paris Sud, Orsay, France).Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: