XB-CLONE-3917381
Clone Name: IMAGE:7091285
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Gene:
upk1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653597
[+]
taattaatccccccccccccccgaggtgtttcccatctctcaggcaggatagaaggtttattatacgtaagaaaatgaaggaggactcaggcgttcggtg
ttaccagtccatcatcatcttcggcaatgtggtccttgggctctgtggcgtggccctgacagccgagtgcatcttctttgtgtcggaccagagtggcatc
taccccctgctggaggcgaccaacaacgccgacatatttgccgcagcctggattggcatctttacagggttctgcttcttcatcttgtccatcgtcggaa
tcattggcatcatgaagtcgaacaggcgaatgctgatggtgtatctcatcctgatgttcgtagtttatgccttcgaagtggcctcggccatcacggctgc
aactcaggaaaactttttcattccaaacctcttcctgaaacagatgttggacttctaccaaaatccaaacccaaccaacaacgacaacctttggaaaatt
aatggagtcaccaacacctggaaccgctttatgcttctgaacggctgctgtggagtcaacggaccccaagactggcagacctacacatcggtgttccggc
agtctaacagcgattctgcctactcttggccacagcagtgctgcgcaatgaacagcctcgggcaaccgtaaatctggagcctgtaaacttgggtttctca
tatgtcacct
3' EST: CN315489
[+]tttttttttttttttttttttttttttttttttttataaactataaactttaatgcatatttaaaaagaaaaaaaaaacaaaaaaacataaaacacccca
aaaaaactctaattggcaggattgtgtaactcctattaaaatgcatgtgtctagagatgagggcggggcattccaaaagggcggaggcaaatggtcttct
aagcttagcccgacgggagcaaacttcacattctacaactccaaatcatttgaccaattttgcccccagtaacactatatatacacagggcagttgtgtg
cccaacggggctcctcctccttccaaacatgtccatgggccaataaaacaaatgttagtaaaagcttggagtttggcccaaaagggatgtccttcatgtt
ggccaagttgtcgtaaaccttcgttttttcaaaggtttaggaaaaaagtcagtctgagctgccttaatactcaatacgggtccagtaaagcatgcttccg
aaaaaaacccaaaatgtccagcaaaaaatggaaaatccaaaccaggccactccccaggcatgtcgggtcattgggccagctatctggtcgtagcaaccat
tcagattgacatatggagaaaccccaagtttacaggcatccagatttacgggttgcccgaggctgttcatggcgcagcactgctgtggccaagggtaggc
agaatcgctgttagactgccggaacacggatgtgtaggtctgccagtcttgggggtcgttgactccacagcagccgttcagaagcataaagcgggtttca
ggtgttggtggactccattaaatttttccaaaggttttcccttggttgtgttggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: