XB-CLONE-3917367
Clone Name: IMAGE:7091853
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654505 [+]
taattaatcccccccccccccctcttccgcctgtttgtctgctgtgaggcagactgtccgtatcggcctaagcccgtcccgtgaagagaatccaccgcca
tctgccacaatggtgaacttcacggtagaccagattcgttccatcatggacaagaaggcgaacattcgtaatatgtcggtcattgcccatgtggaccacg
gtaaatctactctgactgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacagataccagaaaagatgaaca
agagagatgtatcaccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaaagcaaagatgggtgtggc
ttccttataaacttgattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacggatggtgccttagttgtagtggact
gtgtttcaggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgccctgtgttgatgatgaacaaaatggatcgtgctct
tttggagctgcagttggaaccagaggagctctaccaaacttttcagcgcattgtggagaatgttaatgtgattatttccacctacggtgaaggagagact
g
3' EST: CN315576 [+]atacaagctggcagaaggttctaggaagttttttgccatcagcgtttgttgcagacttgctgaactttccatttgcagggtcaaaatacctatcacccca
cagcttcttcatcatgtcctccacttttttggcccgttctgcaggagccaactgaccttccccctttgctgcaaattttgcgacatacatctctgcaaac
tgttttagtgtgaaagcccagccatgcagaccagagccaaaaccaactgtgccaagaacagggtcaatcatgatatttcccatgggtccagtctctcctt
caccgtaggtggaaataatcacattaacattctccacaatgcgctgaaaagtttggtagagctcctctggttccaactgcagctccaaaagagcacgatc
cattttgttcatcatcaacacagggcggatgcgctctgcaatggcttgacgaagcacggtttcagtctgcacacagacgcctgaaacacagtccactaca
actaaggcaccatccgtgacacgaagagcagcagttacttcagaggaaaagtctacatggccaggggagtcaatcaagtttataaggaagccacacccat
ctttgctttgcttgataaaagccaagtcgttctcagagagctcatagaagagagagattgctgttgatttgatggtgatacatctctcttgttcatcttt
tctggtatctggtaaacgagtctcaccaaccctagcaaaaccaatgaacccgcccttgcataccagtgaagtcattcaaaataaaatttcccggggttcc
aattggggcaaagaacccaacatttttaccaaaaggtttcccctttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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