XB-CLONE-3917319
Clone Name: IMAGE:7091163
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Gene:
sp9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653893
[+]
taattaatcccccccccccccgagtggagcagcgcgttggaggcagtaggtggaacctcagtcggtacaagttgtgtgcggagaagggagcaccctgcct
ggcagcacctcttccccacccaggcaccctgatctctgcccagggccaagggacgggctatggctacctctatacttggggaagaaccgagatttggtac
cactcccctagccatgttggctgccacctgcaataagatcgggaacaccagccccctgagtagcctgccggagtccagcgcctttgccaaaggaggtttt
catccctggaaacgctcctcatccagctgcagcctgggctctagcctggggggattcgcagtagccaccagccgggcctccggtgccctaacagggggaa
cagccacagccaacagtgcgttctgcctggcctccacctccccaacctcctccgccttcagcagtgactatagcggactcttttccagcgcctctggggt
gtccccgcaggaatcagccggccagtcagctttcatctccaaggttcacgcctccgcggagggtctgtatccgcgggtgggaatggctcacccatatgag
tcctggtataagtctggcttccactcggacgtgtctggcggggcttcggcctggtgggatgtacacgctgccggccccagcacatggctggaggtgcaga
cacgcaaggggcct
3' EST: CN315856
[+]tttttttttttttttcatgatcagaagtctttaatacgaaaatcaaaatatgaccaggatttttttttttttataatacagataaacgcatttaaatttg
attttctttctttttatttttttttttttttaatttacgcgtctgagtgaaatgttaaatcaacattttaaatatctcttacatatatatatatattgac
tttcacaaagtgcatcaaaaaagaaagaaaaagaaatatcctgaatttcaggaatccacgaaaaataaaactattggaataaacgtttacaatggggaaa
atgaataaaaatcaacacgacctttctgaaacctatttttatacagaatataggaaatgcagataaatcgtcccaaatatttccctcggtttttttgttt
catcttctcaccttaatctgcgtttaaagatgaattgaatttattgatcataaaaaaaaaactgttaatagatctcagtggaggatttagtgtcgtaatc
cacaaaggaatcggaaggggtttctggtctttttaatcgctgtaatagaaagaataagggggttcccagtctctgagccccgataattgcgtctattgct
ctgaatctagaagtcctgcgatattttctagtttcattttgcctttgccagtacatagagtccagtgcactcgagcaacaagtagctatgaatccccccc
aaaaattcagtcgcatctctaaggcgtccgtatgcgtttttgtcgccacaactggctgcacccccaggaagaagtccatgtggccccagaggggggcgcc
ccccccccctaggagtcatttccagaaatccttttccggggcctac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: