XB-CLONE-3917315
Clone Name: IMAGE:7091105
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Gene:
rps3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653760 [+]
aaagtaattttccccccccccgccccttctctcccagctctgcaagatggcagtcggcaagaacaagaggctgactaaaggtggcaagaagggtgccaaa
aagaagattgtggaccccttctccaaaaaagactggtatgatgtcaaggcaccagcaatgttcaatatccgcaaccttggaaagactcttgtcaccagaa
cgcaaggaacaaaaattgcctctgatgggctgaagggtcgcgtctttgaagtaagcttggctgatctgcaaaatgacgaagtggctttccgtaaattcaa
gcttatcacagaagacgttcagggcaagaactgcctgaccaacttccatggcatggatctcactcgtgataagatgtgctccatggtcaagaaatggcag
accatgattgaagcacatgttgatgtgaagaccacagatggctacttgctccgcctgttctgtgttggattcactaaaaagcgcaacaatcaaatcagaa
agacttcctatgctcaacaccagcaggtccgtcaaattcgcaagaagatgatggaaattatgacccgtgaagtgcaaacaaacgaaccttaagaggttgt
tataaactgattccagacagcataggcaagatattgaaaggcctgccgtcatttacctctccacatgttatgtcgtaaaggaaatgctgaaaagcaaagt
tgact
3' EST: CN315876 [+]tttttttttttttttcggtttattttacagtttttaaatcagaattctttaactcagaccgattcctgcactgggggttcatacccatcagcacgttcca
ctttggcaccagtctcgtctcctgctggttttccagctcctccaccctcgccatgaagttccattagtttgcccagctcaaactttggcttcttcagcat
cttcactttacggacataaacatcgtggagaggataaattgactggcaggccttttcaatatctttgcctatgctgtctggaatcagtttattaacaacc
tctttaaggtcgtttgtttgcacttcacgggtcataatttccatcatcttcttgcgaatttgacggacctgctggtgttgagcataggaagtctttctga
tttgattgttgcgctttttagtgaatccaacacagaacaggcggagcaagtagccatctgtggtcttcacatcaacatgtgcttcaatcatggtctgcca
tttcttgaccatggagcacatcttatcacgagtgagatccatgccatggaagttggtcaggcagttcttgccctgaacgtcttctgtgataagcttgaat
ttacggaaagccacttcgtcattttgcagatcagccaagcttacttcaaagacgcgacccttcagcccatcagaggcaattttttgttctttgcgttctg
gtgacaagagtcttttccaggttgcggatnatgaacattgctggtgccttgacatcataccagtctttttttgggaaaggggtccacaatctttcttttt
gtcaccccttcctgccccccttttagtcagccctcttgtttttggccaactgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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