XB-CLONE-3917299
Clone Name: IMAGE:7092007
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Gene:
tfap2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653812
[+]
taattaatcccccccccccccgctcttcggttcacgctcgatttccaagcattcttcctttattaagtatcggtgtaatattaatagtcatgaatatgag
ggctgcaaggctggcagcctgcgcctgttagtaggaaaagcctgcggagctgcatccaggaacagtgtctaggggacaaggattaaggaaacccagaaat
ccaaactttgcctaaaacatccctccctgaatagcaattgtgctctcaacccaaaatgaagaagaatttcctccacatctgatggattatatatgcttat
tatgcctacagagccttggtccttatgattttgtagggaccagtttctagagctgggattttattttcattttttttccacttctctaaatttttttttt
cacatttcttctttgatcaacgttttctttctgatttctcttcttcaatttgtatatatactctgcccagatgttggttcacagtttttccgctatggat
cgccatgatggcaccagcaatgggactgctcggttaccccaactgggcacggtgggacagtctccctatgccagtgcccccccactctctcacactccca
atgctgacttccagcccccctacttccctccgccctaccagccaatatacccccagtctcaagatccttactcccacgtcaatgacccctatagtcttaa
tgccctcctacccagcacaaacacagcacc
3' EST: CN315961
[+]ggctccagaatgggatttggtctggagttacccaagggggatctgtcctgagacagcaaatctgtgaattctttacaaatctgtttggtggcgagaagca
tgttcttcctcgttacttgctcgttcgggtcagaatgttgccggttgacatattcccctattgctttggcaggaaattctgtttcgcacacatacccgaa
atctctggctagatggactgcttccccctccactagggatgtgaggagcgtgacattggcagctttgcgtcttcctgccggcaagtttaaccccatcttg
tccagcttctccctcagggatcttcctccgtttttagattttgccctccttagtactccccccagcagggaggcattgaggcactcgggcggggacagcc
ttctctggacttcggcaactgtcaccttgtactttgaagtggagctgagcagagacaggcgtccaggtactgagcagaacacctcgttgggattgactac
tcccccgaagagattgtctttcttaattacagtttgatctgggatgttgatactcgggtcctcaatatgcgatacgtcttcaattccgtgaggtatggag
tgtaaaggcaggtcccccagtcctgagctgagtccatgaggcccatgtagtaaatcttcgtgctttctgtattccctccgtgggtccaggccagagagct
ggttgcgtatcccccgatgtgtgtgtagcagactagtctcttggctctgtctctgtcccggccagcctggggggctgtggttgtggctggggtagggagg
gcattaaaaataatagggggtcattttaccttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: