XB-CLONE-3917205
Clone Name: IMAGE:7091055
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Gene:
slc25a5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653752 [+]
tatttaatccccccccgccccagccctaggaagcttttggtgctgcttcttgagcctagcatctgctaacgtaacaccggcatcaccatgactgacgcag
ccatctcttttgccaaggacttcttggccggcggtgtggccgcagctatctccaagaccgctgtagcacctattgaaagagtcaagcttctactgcaagt
ccaacatgcaagcaaacagatcaccgcagacaagcactacaagggcatcatggactgtgttgtcagaatccccaaagaacagggtttcatgtccttttgg
cgtggtaaccttgccaacgtgatccgttatttcccaacccaggccctcaacttcgccttcaaagacaagtacaagaagatcttcctggacaatgtagaca
agaagacccagttctggcgctactttgctggcaaccttgcttctggtggtgctgctggggcaacctccctctgctttgtctacccccttgactttgcccg
tacccgtctagcagctgatgtgggcaaaggcaatgctgagagagagttcaagggccttggtgattgcttagttaagatctccagatctgatgggatcaag
ggcttgtaccagggattcaacgtatctgttcagggcatcatcatctacagagctgcttattttggcatctatgatacagctaaaggtatgcttccagatc
cccagacacacacattac
3' EST: CN316694 [+]tattttttatttcctcaaacctaagttacttaaaaacatgaatgactgaaataaaaaaataaaggagcgttgagtcccactgaatcagcctgtcactgcc
aaagggccaaaaactgccaaggaggcacagttgcaggcactaaaagcagtaagagacttaaagggaatgatttattaatgtggaaagctggtgagatgta
atagaactgatcccaaaataccagccagtaaagattaccagctatattttataaataagggtgtgtttgacaaaaaaaataaagaaggtccatgaatgtt
caaggtatgttacataatacagcatgccaggtcacagacatctcaacgaggacaagtttagatgtatttcttcagctcatcatacaacaccagcacaaaa
gcgccacccattcctctgagaacattggaccaggcacccttgaagaaagccctgctgccctcatcccgtgcaatcttcctccagcagtcaattgttccac
tgtacatgatgtcagctcctttccttccagactgcatcatcatacgacgacgtactgtgtcaaatggataggagacaaatccagctactgctgttacagt
ctgagcaatcatccagctgatgataatgtgtgtgttcttgggatctggaagcatacctttagctgtatcatagatgccaaaataagcagctctgtagatg
atgatgccctgaacagatacgttgaatccctggtacaagcccttgatcccatccagatctgggagatctttactaagcaaatcaccaaggcccctgtaac
tctcctctcagcatttgccctttgccccaccttcaaccttgcctaaacgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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