XB-CLONE-3917159
Clone Name: IMAGE:7092043
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654539 [+]
taattaatccccccccccccccacccagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgagg
agactaccgccttggtgtgcgacaatggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtcg
cccccgtcaccagggtgtcatggtgggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggtatcctgaccctgaagtacccc
attgaacacggtatcatcaccaactgggatgatatggagaagatctggcaccacaccttctacaatgagctgcgtgtggcccctgaggagcaccccaccc
tgctcactgaggccccactgaaccccaaggccaaccgtgaaaagatgacacagatcatgtttgagaccttcaacgtcccagccatgtatgttgccatcca
ggctgtgctgtccctgtacgcttctggtcgtaccacaggtatcgttcttgactccggtgatggtgtcacccacaatgtccccatctatgaaggttatgcc
ctgccccatgccatcatgcgtctggacctggctggtagagacctcacagattactcatgaaaatcctgactgaacgtggcactcttttgtcacacagctg
aacgtgaattgtccgtgact
3' EST: CN317053 [+]tttttttttttttttaacgtaaccgcttgtgttttatttagaaagtaataaatatgatggagtgtaaacaaactgtacagtgactgtccaggtcttgttt
tcttcgactttgaggtactttgtatgtgttgcttggaggagtgtgcgtaatgtttagaagcacttcctgtgcacaatggaggggcctgcctcatcgtatt
cctgtttgctgatccacatctgttggaaggtggacagggaggccaagatggagccaccgatccagactgagtatttacgctcaggtggggcaatgatctt
gatcttcatggtgctgggagccaaggcagtgatttccttctgcattctgtcagcaataccagggtacatggtggtaccaccagagaggacattgttggcg
tacaggtccttgcggatatcaatgtcgcatttcatgatgctgttgtaggtagtttcatggataccagcagattccataccaatgaaggaaggctggaaga
gggtctcagggcagcggaaacgttcatttccaatggtgatgacctgaccgtcgggaagctcatagctcttctccagggaggaggaagaggcagcggtggc
catttcattctcaaagtccaaagccacatagcacagctttttcttgatgtcaccgacaatttcacgttcagctgtggtgacaaaagagtagccacgttca
gtcaggaatttcatgaggtaatctgttgaagtctctccagcccaggtccgacgcatgatggcatgggggcaagacttaactttcttagatggggaacttt
tgggggtgaacccatccccggaaattcaaaaacaaaacctgtggggtacaaaccaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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