XB-CLONE-3917149
Clone Name: IMAGE:7091883
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654507 [+]
taattactttccccccccccccgacccagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgag
gagactaccgccttggtgtgcgacaatggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtc
gcccccgtcaccagggtgtcatggtgggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggaatcctgaccctgaagtaccc
cattgaacacggtatcatcaccaactgggatgatatggagaagatctggcaccacaccttctacaatgagctgcgtgtggcccctgaggagcaccccacc
ctgctcactgaggccccactgaaccccaaggccaaccgtgaaaagatgacacagatcatgtttgagaccttcaacgtcccagccatgtatgttgccatcc
aggctgtgctgtccctgtacgcttctggtcgtaccacaggtatcgttcttgactccggtgatggtgtcacccacaatgtccccatctttgaatgttatgc
cctgcccctgccatcttgcgtctggacctgtctggtaaagacttccagatttcctcttgaaatcctgatgactgtgatactcttttgcccacacttgaat
gtgaattgtcgtgactcagg
3' EST: CN317115 [+]tttttttttttttttgtacgtaaccgcttgtgttttatttagaaagtaataaatatgatggagtgtaaacaaactgtacagtgactgtccaggtcttgtt
ttcttcgactttgaggtactttgtatgtgttgcttggaggagtgtgcgtaatgtttagaagcacttcctgtgcacaatggaggggcctgcctcatcgtat
tcctgtttgctgatccacatctgttggaaggtggacagggaggccaagatggagccaccgatccagactgagtatttacgctcaggtggggcaatgatct
tgatcttcatggtgctgggagccaaggcagtgatttccttctgcattctgtcagcaataccagggtacatggtggtaccaccagagaggacattgttggc
atacaggtccttgcggatatcaatgtcgcatttcatgatgctgttgtaggtagtttcatggataccagcagattccataccaatgaaggaaggctggaag
agggtctcagggcagcggaaacgttcatttccaatggtgatgacctgaccgtcgggaagctcatagctcttctccagggaggaggaagaggcagcggtgg
ccatttcattctcaaagtccaaagccacatagcacagcttttccttgatgtcacggaacattttcacgttcagctgtggtgacaaaagatagccacgttc
agtcagggattttcatgagtattctgtgaggtttctacccacccgggtccaaacgcatgatggcctgggggcaagggcataaccctttaaaaatgggggg
aatttttggggtgaaaccccctccccccgaaattcaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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