XB-CLONE-3917075
Clone Name: IMAGE:7091794
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Gene:
npm1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654270 [+]
taattaatccccccccccccaaacccccccgagaatccgtccgtgccgctgtaaagtaaagggggtacatctgaattgaattggaacaaaatggaaaact
ccatggatatggatagcattgctcccctgccccctcagaattttctctttggatgtgaacttaaagcagacaaaagagaatattcatttaaagttgaaga
agatgaaaatgagcaccaattgtcattgaaaacggttaatgtgggtgcatctgtctaagattaactgcatgttgtttgaggcagaaggaataaattacga
agggaaaaccattaaaatttcatttgcctcattgatttcttctgtgctaccaacggtttctctagggggatttgagataacacataccatgaatcttgct
gttgaaatctgggtcagggacgaaatatagcaatgggcagcgtcatgtgtctttaaaaaatactttatacccttaagaagtatatgaagaatatgatgtg
ctccattcataaatagcatgaagacagaatcaaatgccgtatctaaggccttccaataacactctcacggtggagatgacaagttgattcctccatgatc
atacatgtgactacttaatataagttaatatatttatcagagtataaaatctgcaaatttaattacctcacctagtgtcacaatgcaaatc
3' EST: CN317637 [+]tttttttttttttttgtttttttttttttttgttttttttaatgtaagaacaactttatttaataacgcatgcaatcttacatttcaaagaccgggccca
acagttaaaaaaaaaaaccaacaacaagaacaaacaacttgataacaagaggcaggtttattttgagtctttcagggactgtctccatttgaaaagatct
tgaattaccttctgattttctgccctaaagcaattcttcacatagtttgcaaactttacttccactttaggaagaacagtgcccttttctaatgaagttt
gcatctttgcttttatttcatcaaaagacaaaggcgtttttggtgtctttggtgtttgaggctttgtatcctgctttcctttctgctctggagtttttgg
agtctttagtggtgtgggtaattcaaaagccttcccattgtgatttaacttttgagctgcctttggttttgtaggcaagtcagtctttttaacaggtgtt
tcatcctcttcatcctcatcttcgtcgtcctcctcctcatcatcgtcatcatcatcatcatcctcgtctgaatcagcctcgtcctcttcctccagacgtg
tttttttccgaggaacctttgatgcagcatctgatgccgtcctttttgcattttttggactgggcacatcatgttcctcatcttcctcatctgtggaatt
caagatcttctaaagccacaaaatgctgccccctgacatatactggccctgtacccaaatttcaccgcaagattacgggggggggttatcccaaaatccc
ccaaaaaaaaccgttgggtggcccaaagggctttaaaggaggcaaagggtaaattttatggggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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