XB-CLONE-3917063
Clone Name: IMAGE:7091344
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Gene:
ddx20
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654365 [+]
taattaatcccccccccccccggcatttgttgcacggcaagatggcggcgcacatgctgggatctcggccgcgcactgaggatgtttcgcagggtaccgg
ggatactacagattttaaatcgctgcttctatcccgtcctgtgctggatggactggaagcagctggcttcaacaagccgtccccgattcagagcaaagcc
attccgttaggcaggtgtggcctggatcttatagtgcaagccaagtcgggaacaggaaaaacatgtgttttctctacagttgcactggattccttgatct
tggaaaatactgcaactcaggtgttggtattggcccccaccagagaaattgcagttcagatccattcagtcatgttatccataggctgtcgcatggaagg
gctggaaagtcatgttttcataggagggacaccactgccacaggacaaaattcggctcaagaagtgccacatagctgttggctctcctggtcgaataaag
cagttgatagaaattggttaccttaacacgtccagtgttcgtctttttatcctcgatgaagctgataaacttttggaagaaggaagtttccaggagcaaa
taaactggatatattcctctcttcctgnnctacaacaatgctagctgtttcagccacttac
3' EST: CN317734 [+]tttttttttttttttggcaaaatgaatcacttattcctttaataagcattacactgttgaaaaatgagtaggtcaatagtcagctaagactaaaccctcc
aaatacagttgctgactgaaacagtgaaatacttgaaggtagagaaggtatataagtaatcatgcagaatttacaacataatgaaaaaaagaaatatata
gcattttcaagctggtttactattgtgtattaaaccaattttatgtatttgttggagttttgcaatttctgttttaaaccagaaaaatgtgatcaccagt
gaatggtgaaggttgtttaaggcatagaagaatattaaaatgtaaaccataaaccactgccttattattaacataatttgagtttgtcatggctttagca
tttcatggaggtacacagattgcatgtgatatgcagacatccagcttgaacgcttgtacagattacggtagtaagaatttgatacagtagaatggtatgt
ttgccatgcttgataataggacctccagtagtcagcatagctcgtggtgccatatggaaatctactagggtttctatattccaactggtacaaatcatct
tgtcttttcgaacagagttcacgtcttgacacaggtgttgtgttctttctccgtcttctagatttggtatttgctcacaaaaggggtttttacctttttt
ttttttgagcttttctcaccttttccttctttttgtcaagttaaaaatttttaattgtgtttgagtttaacaaaaaagtaaggtttaattttcccttttc
cttttttaatcttctgaaatattaaatattaaaaaataaaaaaaagggaaacttcttttgggccttcgggaaataaatcaaaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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