XB-CLONE-3917055
Clone Name: IMAGE:7091082
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Gene:
phf14
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653962 [+]
taattaatcccccccccccccgggtacaacaggggaagcttgcagttgatggctttgtgggaggtgtaagtagcggtcctggactggggactgtgttagg
aaacgaggggctgttgggcagtgtgcgtctgcttaaataagagggtgaaatccgagctccccccccaagcctatccaatccccgtacggctctacgaaga
cgagaactgtgacaacacgagcagtacatacagctctgtaggggaataaagacagacaagctatttacctccattccgaactctgaagctcactgacagc
agggagacgagtaacacaatggaccggagttcgaaaagacgacaagtgaagcctttggcaacttcccttcttgaagcattggattatgatagctcagatg
acagtgattttaaagttggagatgcttcagattctgatggaagcttgaacgggagtgacgatatgtcaagggatagtgctgatggctctggaagtgagtc
agaagaaaatataatggagagaataccagctgctgcagtggaaacagaagaaaaaagacctgaagaagcgttgacatgctcagctggaaatgaacaggga
atttagatctgagtaaaaaaaaaaaaaaggatccactatttctagacggccgccaccgcgtggacttggccgtg
3' EST: CN317805 [+]tttttttttttttttactcagatctataattccctgttcatttccagctgagcatgtcaacgcttcttcaggtcttttttcttctgtttccactgcagca
gctggtattctctccattatattttcttctgactcacttccagagccatcagcactatcccttgacatatcgtcactcccgttcaagcttccatcagaat
ctgaagcatctccaactttaaaatcactgtcatctgagctatcataatccaatgcttcaagaagggaagttgccaaaggcttcacttgtcgtcttttcga
actccggtccattgtgttactcgtctccctgctgtcagtgagcttcagagttcggaatggaggtaaatagcttgtctgtctttattcccctacagagctg
tatgtactgctcgtgttgtcacagttctcgtcttcgtagagccgtacggggattggataggcttggggggggagctcggatttcaccctcttatttaagc
agacgcacactgcccaacagcccctcgtttcctaacacagtccccagtccaggaccgctacttacacctcccacaaagccatcaactgcaagcttccctg
ttgtaccgggggggggggggggggattaattaanttnaagtgaaaaaagaaaaagtagtttgtatttgggctgggaaagaaaagagggggcattggaagt
ggggatgggaaaattgtggaaagaaaagaaaaaaggaaggtaattttaattttgtaaataaaattgaaagttgtataaataaaaagggtaaggtattaaa
ttgtaagngtgtttttaaaaaaaaaaaaagggggggggggttaaaaaaaatataataaggggggaaatta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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