XB-CLONE-3917049
Clone Name: IMAGE:7092130
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Gene:
lsm12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654337 [+]
taattaatcccccccccccccgacactcttgactgcctctgttaggaactgtgacgcttgtgagggctgcctctctgatcataccacaaaatgtctgaca
aaccagatatggctgagattgagaaatttgataagaccaagctgaagaagacagaaacacaagaaaagaacccactcccatcaaaagaaacgattgagca
agagaaagcaacttcataatgatcccccatcacccagatatgcactgtacattccacaagcattgccttcttattttactttagctgtttaactttgtaa
gaaataactcaaaatacaaagaatttggagaacatgttctgtgctgccctgttacaccaaaagaaaggaataagaactactgaacacggaatgaaggcgc
tgcctttccatctgcctgtctggctggctttggttctggtggcattgaaagatctttcttgattatttaaggagaaccagtgtggaaataacagaactaa
agtaacatgctctgatgtgggtgggatgaaagctgcaccaaggtccagcgagctgtaaaatacggtcaaatcgagtgccatttatttgttcaaattaatt
gaatattggaatgcacaatttttttaaatgcaataaaaatgaaaacataaaaaaaaaaaaaggatccctatttcaaacggccccacgcgtga
3' EST: CN317838 [+]tttttttttttttttatgttttatcatttttatttgcatattaaaaaaaatttgtgcattccaataattcaattaattttgaacaaataaaatggcactc
gatttgaccgtattttacagctcgctggaccttggtgcagctttcatcccacccacatcagagcatgttactttagttctgttatttccacactggttct
ccttaaataatcaagaaagatctttcaatgccaccagaaccaaagccagccagacaggcagatggaaaggcagcgccttcattccgtgttcagtagttct
tattcctttcttttggtgtaacagggcagcacagaacatgttctccaaattctttgtattttgagttatttcttacaaagttaaacagctaaagtaaaat
aagaaggcaatgcttgtggaatgtacagtgcatatctgggtgatgggggatcattatgaagttgctttctcttgctcaatcgtttcttttgatgggagtg
ggttcttttcttgtgtttctgtcttcttcagcttggtcttatcaaatttctcaatctcagccatatctggtttgtcagacattttgtggtatgatcagag
aggcagccctcacaagcgtcacagttcctaacagagcgacaaaaaattgtcggggggggggggggggattatgggatttggtgttaaaagaaaaaatttt
ttgaattctgggcccggataaaacgacggggtggattaattggggggaagggcccatgggacaaaaaccaaaacgggggggaaaacctttttttctcccc
ctgagcctccttattgtttgaaaactctcgggtttgggaggaaaaatgtgtccccccgggtttcagaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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