XB-CLONE-3917011
Clone Name: IMAGE:7091264
|
Gene:
sub1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK653717
[+]
taattaatcccccccccccccgttccgttgagaagcgatcccagaagcacacgtggtgctagcggacagaatgccaaaatcaaaggagatagtgtcctca
agctcatctggaagtgattctgatagtgaagttgaccagaaggtaaagagaaaaaagcaaccacctccagaaaaagaaaagccagtaaagaagcagaaaa
caggggaaagttctaagggtggtgcttcttcaaggcaaagcaatgcccctgaggataatatgtttcagattggaaaaatgagatacgttagcgtaaggga
cttcaagggaaaagtcctgattgacgtcagagaatactttatggatcaagcaggagaaatgaagcctggaagaaaaggtatttctttaaatcctgagcag
tggaaccaactgaaggagcagatgtcggacatcgacgacgcaataagaaaactgtagagttctacccagctttgaagaaaccaagttttcattttaagca
ctctttttacattcgctgttttacttttttttttttttttttttttttattacgcgctattgtacatttggattgtaaacaatttgtcaaatattacacg
tctgcgctttacatggcattggaatttcaccacgctactatgaaaatatttttgttaaaaagtttccataaaaatatcatct
3' EST: CN318076
[+]tttttttttttttttcgttttaaagaatgatatttttaatgggaaactttttaaacaaaaatattttacatagtagcgtggtgtaaattccaatgccatt
gtaaagcgcagacgtgtaatatttgacaaattgttttacaatccaaatgtacaatagcgcgtaataaaaaaaaaaaaaaaaaaaaaaaagtaaaacagcg
aatgtaaaaagagtgcttaaaatgaaaacttggtttcttcaaagctgggtagaactctacagttttcttattgcgtcgtcgatgtccgacatctgctcct
tcagttggttccactgctcaggatttaaagaaataccttttcttccaggcttcatttctcctgcttgatccataaagtattctctgacgtcaatcaggac
ttttcccttgaagtcccttacgctaacgtatctcatttttccaatctgaaacatattatcctcaggggcattgctttgccttgaaaaagcaccaccctta
gaactttcccctgttttctgcttctttactggcttttctttttctggaggtggttgcttttttctctttaccttctggtcaacttcactatcagaatcac
ttccagatgagcttgaggacactatctcctttgattttggcattctgtccgctagcaccacgtgtgcttctgggatgcttctcaacgacggggggggggg
ggggaatttttaaccctagtggaaaaagaaaaaataccttgttttccgggcccggaataaaccgaggggcgattggacccggggaggggaacccccgtgg
gaaaaaaaaggcaaaaagggaattttattccttaattgtttccccaacatctcgttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: