XB-CLONE-3917009
Clone Name: IMAGE:7091301
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Gene:
stam2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653605 [+]
taattaatcccccccccccgccctatcccagctctggtcatgacgcagtttcaaactccccatatttaaaccagagttccgccctccattcagccgcagg
gcttcctgcttacagccagccaatgggcatgtcgtccgatgcatcagcctatcaaaacactacatctgctctgccaccgggatactccgtgacggctcct
ctccattcaatcgctccgcaaccccagaactactaccagcagcctctgctctgaaacagaacccctgagagtgaatacaagtcggatgtgtgttaattcc
cagtcttgtaactcatatcaccggctttcatggtaaacacagacacataggaaatatgcattgttagtctaacggaccaggaaccagcctgagattccaa
caatacaatggtgctccattccctctgggataaatgcactgctctccttttccctagatcccaagctgcctccgtacaattagcctgatcatactctgcc
tcgttactaatgagctgccggtgcataggggtacacgctaattagcgcagccttcattgtattgcatacaaaggtttataaataggaaagaaaagccttt
ccttggcaagtagaactgcccactgacttctcacttctgccataaggtcagtttccatagagaactgatggacggatggtgatggaagttctagttcaac
agcctgtgaa
3' EST: CN318077 [+]tgtttgacttacagggaaacatagcgccatctataggctacacaaggcatccccttgcagggcactaagctctgctccaatgagacattgcctccaatgc
tgagagagtacaataatctaactaaccaatcagatcactacacacaagatcagctatgtagtgaatgatgttattggttagcaccacaccagtatgggag
ttatgtaatgaaacacccagtggactgagaaaaagaaacattgatgtgatgaagaggtctgtacattggactctaaggtgctatcagttctatccataat
cagcgtatttatctattttatattaaaaaaatgtatcattaagaaagacacacaagccccatccagcagctggcacggcgttgggcatcacgtggccgct
tctgccgtttgccgtctattcagatggtcgtcaaggaaaataaagcaataataataataaggcggctttaatatgggccaaacccagggccacgacacag
aaatggggcccaacacaagctttagagaacataacaagcaaaaaatatgtttcaatatagaaaagaggtgcatttgcagcatggggtattaggtgtaata
gggtgagattccaacaacactcgggtattctgcacattatcgagggtaaaggagatgtcatgacatgaacagcccggatgaaacgcatgttcctgaccag
taaaggggaacttagcctgtacctatgtacgctgggggtgtcagtgactgcattatcctgttaggagagtactggggaaaggttcttctaccagccccgg
gcaattaaaggggctggcccccccggggccatgtttccggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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