XB-CLONE-3916935
Clone Name: IMAGE:7092011
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Gene:
tnni2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653814
[+]
taattaatccccccccccccccagtcccacccctgcttcatagagagggctcagtctacgagggtcaggtatcacagaaaccgccaagatgggtgaagat
gacaagaaatccaagatctccaatactcgcaaaacccacctgaagagcttgatgctccaggtggccaaaactgctcttgagaaagaagaagctgaaattg
cggcagagaaggaagcctacctacaagagcactgcccacctctaagccttcccagcaacttatcagaactccaggatttctgcaagaagatgcacgccaa
aatcgatgtggttgatgaggagaggtatgacatggaagtcaaagttggaaagagcaccaaggagctggaagacttaaaccagaagatctttgatctgcgt
ggcaagttcaagaggcctgcccttaaacgtgtccgtatgtcagctgatgctatgctgcgtgctctgcttggctccaagcacaaggtcaacatggaactcc
gtgccaatctcaaacaagtcaagaaggaagacactgacaaggagaaggatctccgtgatgttggtgactggcgtaaaaacattgaggagaaatccggcat
ggaaagcaggaagaaaatgtttgaatctgaagctttagcccttaaccaagaccactgccatcttaaccccttagctccaaaaacaccacctaccctaatt
tcctgttactaaagggtttttccctgctgtcctccc
3' EST: CN318570
[+]tttttttttttttttagagaagaaaggaccgtgacgtcacctccagatcaggtatcaccaactttaagggacgtcacaagctttatttacaagtttatgt
tttcacgtccgcataacacaactgctgcaaagccaatacagaggggacccagaaatttgagcccctccttgagtccataacattctttaagaccttctac
aagggtataaagtggagtggtgaaatatccaggagtggggaggaggtaagacttcagtatcccctttgccgctggagagtgagcagcagaggaaataacc
ctctagttaacagagaagttagggtatggtggttgtctctggaagctaaggggtttaagatggcagtggtcttggttaagggcttaagcctcagattcaa
acatcttcttcctgccttccatgccggatttctcctcaatgttcttacgccagtcaccaacatcacggagatccttctccttgtcagtgtcttccttctt
gacttgtttgagattggcacggagttccatgttgaccttgtgcttggagccaagcagagcacgcagcatagcatcagctgacatacggacacgtttaagg
gcaggcctcttgaacttgccacgcaaaatcaagatcttctggttaaatcctccacctcctggtgctcttcccacttttaactccctgtcaaacctccccc
caccaaccacaacgatttttggggggctcctcttgcaaaaatcctgaagttctaaaaatttgcggggaaggtttaaaggggggcaggggcctttttgggg
tagggcttcccttcccttccccaaatttcccccttctttttttttccaaaaaaaaaattttggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: