XB-CLONE-3916927
Clone Name: IMAGE:7091228
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Gene:
eif3m
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653711 [+]
taattaatcccccccccccccgtccggtgcagccgcggcgtgtgatcgggacagcgtgccgctgctatgagcgtcccggcgtttatcgatgttaccgagg
aggatcaggcagccgagctgcgggcttacctgaagtctaaaggagcggagatctccgaggaaaattcagaaggggggcttcacattgacttggcccagat
catcgaagcctgcgacgtctgtttaaaagatgatgataaagatgtggagagtagtatgaatagcgtggtgtctctggtcctgattttggagacggataag
caggaagctttgatcgaaagcctgtgcgaaaagctggtcaagttccgggaaggagaacgtccatcattaaggctgcaactgctgagtaatctcttacatg
gcatggacaaaagtatcccagctcgatacactgtatactgtggtcttataaaagttgcagccacctgcggagccatcatctacattcccacagatctcga
ccaggttcgcaaatggatttctgactggaacctcagcacagagaagaaacacattgtgctcaggcttctttatgaagctcttgtcgactgcnagaaaagt
gacgaagcagcgaaagtgatggtggagctgctcggcagttacacggatgataacgcttcccaggctcggctggacgctcaca
3' EST: CN318639 [+]gaggaggtcaaattttataaagcgtcccaaaaactgctgttttgcactggataaaaaaaagctaataatcatcatcatcataatcaagcgtcggaaatac
tgtagagactgttcttcactttgtttaagttctgcttccaagtgttgagcgtatcatataattgctgccactgctgcttcccgaaggtcctgtgtgtgct
gtgactgacgacaaccctcttctgcgtctggtcgatcttgcagtacaccatctttgttttgacagcatcaataatgaatgcttccacctcgtctgcgccg
atctgcagctcctgctggatagtatcgaatgagatttccttgttgtccacggccattcccataaaggtaaggagcctcatcttctccatgttttgctcat
gagagagacccagtgagtcaatgaaatctttgttgttctggtagaacttgacgtaggaggacagcttggcgctgacaaatatggtcaaaagatcatgaat
cagttctccttccaggaatttgacaggctttaaagcaagaaggtgatcaagcaaaaaggctttgggatctttcagggcccttacgatgcacttgtgagcg
tccagccgagcctgggaggcgttatcatccgtgtaactgccgagcagctccaccatcaccttcgctgcttcgtcacttttcttgcagtcgacaagagctt
cataaagaagcctgagcacaatgtgtttcttctctgtgctgaagtttcagtcagaaatccaatttgcaaacctggtcaaaatttggggggaaagtaaatg
aaggggttcccccgagggggcttgcaacttttttaaagaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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