XB-CLONE-3916907
Clone Name: IMAGE:7091341
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Gene:
akr1c3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653612 [+]
taattaatcccccccccccccggcagtaaggatctgatcgcttacaccaaccatggcactgcacaaagactcctacgttgagctgaatgatgggcacaaa
atgccagtgattggatttggcacctacgcccctccaaagttccccaagagtttggcagaggagggcaccaaagtggccattgacgttggataccgccata
ttgactgcgcttttctctatgggaatgaggaggaggttggacgggcgatcagggcaaagattgcggatggtaccgtgaagagggaggacgtgttctacac
tgggaagcactggtccacgtcccatacccccgagagggtccggccagccctggagaaatccctgaaagaccttcagttggattacatggatctgttcatc
atccatatgcccatggagttcaagcctggagatgatctctttccggcggatgaaaatgggaaatttatttaccataacacggaccttagagatacatgga
aggctctggaaaagtgcaaagacgcagggctggtcagatccatcggggtgtccaattttaaccacaagcagctggagctgatcctcaatatgcctgggct
gaatataaacccgtctgtaaccaggtggaatgcacgtctacctgatcagacaaactgctggagtctgcaagtcaaggactcgtgttgtgggatcagcgta
tggggtcagcaggatgac
3' EST: CN318774 [+]tttttttttttttttaggaaactaattcaatactttattgtctaatatggaggcaatggggagaggagggggcagtaacaaatgtggatcttctgctcat
tgcaaaaacggctgtatctgtcagtggaaaaatcaggaaccaatcagtattcctcactgtacggatatttcgggtggtcccaccatctggaagtgtccat
gtaacccatgtttctgttcagcccatccatgcttctcatgtcttccgcgtccagctgaaagtcaaaaacctggaaattctgctggatcctggctggggtg
aaactctttgcaaggacaaccactcctccctggaagtggtaccccattgctacctgaacgggggtccggttgtgcttcttaaccatctcagtgagcgcaa
ggtcttccaggaacacaggaatgctcccttctatccatccctcatccctgctggaccccaataccctgtatcccaccaacacgatgtccttggacttgca
gaactccagcagtttgctctgattcaagtagacgtggcattccacctggttacagaccggtttatatttcagcccaagcatattgaggatcagctccagc
tgcttgtggttaaaattggacaccccgatggatctgaccagccctgcgtctttgcacttttccagacccttcatgtatctctaaggtccgtgttatggta
aataaatttcccattttcatccgccggaaaagatcatctcccagcttgactcatggggcatatggatgatgaacagattcattgtaatccacctgaaagg
gctttcccgaatttctccacggctggccggaccccccccccggggggtggggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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