XB-CLONE-3916861
Clone Name: IMAGE:7092104
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Gene:
rpl7a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654594 [+]
taattaatcccccccccccccgcttcctttctctgcccctgtccaccatgcctaaagggaagaaggccaaggggaagaaggtggcacccgccccttccgt
cgtgaagaaggccgaggccaagaaggtggtcaaccctctgttcgagaagaggccaaagaacttcggcattggtcaagatatccagcccaagcgtgatctg
acccgctttgtcaaatggccccgttacatcagactccagcgccagaaggccatcctgtacaagaggctcaaggtcccacctgcaatcaaccagttcaccc
aagccttggaccgtcagacagccacacagctcttcaaactggcccacaagtacagacctgagactaaacaggaaaagaagaaaaggctgctggccagggc
tgagcagaaggccgctggcaagggagacgttcccaccaagagaccccctgtactcagagccggtgtcaacaccgtcaccacccttgtggaaaacaagaag
gctcagcttgttgtgatcgcccatgatgtcgatcccatcgagctcgtcgtgttcctccctgctttgtgccgcaagatgggagtcccatactgcattgtca
aggggcaggccagactgggccgcctcgtgcacaggaagaccctgcacagcgtagcactcacacaggttaacc
3' EST: CN319114 [+]tttttttttttttttggagtttattttttttatttctgtacagaacaaaacaacaagatcggtacatttaaccaagcttggtggccagctcctttgcttt
tgccttctccagtttggcgatgcgggcaacagacttggggcccaggataccaccaccccagtgacgacggatctcatcgaatcggtcattgtagttggtc
ttaactgcttccaccaacttggcgagggcacctttgtcctcagggttaacctgtgtgagtgctacgctggtgcaggtcttcctgtgcacgaggcggccca
gtctggccttgcccttgacaatgcagtatgggactcccatcttgcggcacaaagcagggaggaacacgacgagctcgatgggatcgacatcatgggcgat
cacaacaagctgagccttcttgttttccacaagggtggtgacggtgttgacaccggctctgagtacagggggtctcttggtgggaacgtctcccttgcca
gcggccttctgctcagccctggccagcagccttttcttcttttcctgtttagtctcacgtctgtacttgtgggccagtttgaagagctgtgtggctgtct
gacggtccaaggcttgggtgaactggttgattgcacgtgggaacttgagcctcttggacaggaagggcctctggggctggagtctgatgtaaccggggcc
ttttgaaaaacggggtcaaacacccttgggctggaattcttgaccaatgcccaaattttttgggcctcttcccgaaaaaagggttgaaccccctttttgg
cccccggcccttcttccaaaaggaagggggcggggggcccccctttttccccctggggcccttcttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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