XB-CLONE-3916851
Clone Name: IMAGE:7092127
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Gene:
psmc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654555 [+]
taattaatccccccccccccctttgcttccgctgctggaagtgcgaactcacccggcctgtcggaagcaataaacatggcgtctatatgggatgaaacgg
aggcagatgggttgggcgaagaggttctcaaaatgtccacagaagagattatccagagaactcggctgctggacagtgaaattaaaatcatgaagagtga
ggttttgagagtgacgcatgagctgcaagcaatgagagacaaaattaaagaaaatagtgagaaaatcaaagtgaataaaaccctcccatacctggtgtcc
aatgtcatagagctcctggatgttgatcctaatgaccaggaagaggatggagccaacattgacttagactctcagagaaagggaaagtgcgctgttatca
aaacctcaacacgacagacctacttcttgccagtgattggtttggttgatgctgaaaaactgaagcctggagatcttgtgggagtaaacaaagattctta
cctcattttggagacccttcctactgagtatgattctcgtgtgaaggccatggaagtggacgaaaggcccaccgagcaatacagtgacataggtggtctg
gataaacagatccaggagcttgtggaggccattgtactgcccatgaaccacaggaaaagtttgagaacttgggaatcagccccctaaagtgtcttatgtc
ggccccct
3' EST: CN319203 [+]ggatatccacacgattagtagcagcaatcaccttgacttgtgtgtttggctgaaatccatcaagctgattaagaagctcaagcattgttctctgtacctc
cctgtctcctgctttctcactgtcgaaccttttagtgccaatagcatcaagttcatcaatgaaaatgatagaaggtgctttttccttggccagagcgaaa
gcatctcgtacgagtttggcaccatctccaataaacatctgaactagctgtgggccggcaagcttcagaaaggtagcctttgtctgagcggcgcatgccc
tagccagcagggtcttgccagttccaggggggccgtacataaggacacctttagggggctggattcccaagttctcaaacttttccttgtggttcatggg
cagtacaatggcctccacaagctcctggatctgtttatccagaccacctatgtcactgtattgctcggtgggcctttcgtccacttccatggccttcaca
cgagaatcatactcagtaggaagggtctccaaaatgaggtaagaatctttgtttactcccacaagatctccaggcttcagtttttcagcatcaaccaaac
caatcactggcaagaagtaggtctgtcgtgttgaggttttgataacagcgcactttccctttctctgagagtctaagtcaatgttggctccatcctcttc
ctggtcattangatcaacatccaggagctctatgacattggacaccaggtatggggaggggtttattcacttttgattttctcactaattttctttattt
ttgtctcttcttggctttgcagctcaaggcgttcaccctccaaaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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