XB-CLONE-3916843
Clone Name: IMAGE:7091164
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Gene:
eif3m
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653694 [+]
taattaatcccccccccccatcgggacagcgtgccgctgctatgagcgtcccggcgtttatcgatgttaccgaggaggatcaggcagccgagctgcgggc
ttacctgaagtctaaaggagcggagatctccgaggaaaattcagaaggggggcttcacattgacttggcccagatcatcgaagcctgcgacgtctgttta
aaagatgatgataaagatgtggagagtagtatgaatagcgtggtgtctctggtcctgattttggagacggataagcaggaagctttgatcgaaagcctgt
gcgaaaagctggtcaagttccgggaaggagaacgtccatcattaaggctgcaactgctgagtaatctcttccatggcatggacaaaagtatcccagctcg
atacactgtatactgtggtcttataaaagtcgcagccacctgcggagccatcatctacattcccacagatctcgaccaggttcgcaaatggatttctgac
tggaacctcagcacagagaagaaacacattgtgctcaggcttctttatgaagctcttgtcgactgcaagaaaagtgacgaagcagcgaaggtgatggtgg
agctgctcggcagttacacggatgataacgcctcccaggctcggctggacgctcacaagtgcatcgtaagggccctgaa
3' EST: CN319276 [+]gaggaggtcaaattttataaagcgtcccaaaaactgctgttttgcactggataaaaaaagctaataatcatcatcatcataatcaagcgtcggaaatact
gtagagactgttcttcactttgtttaagttctgcttccaagtgttgagcgtatcatataattgctgccactgctgcttcccgaaggtcctgtgtgtgctg
tgactgacgacaaccctcttctgcgtctggtcgatcttgcagtacaccatctttgttttgacagcatcaataatgaatgcttccacctcgtctgcgccga
tctgcagctcctgctggatagtatcgaatgagatttccttgttgtccacggccattcccataaaggtaaagagcctcatcttctccatgttttgctcatg
agagagacccagtgagtcaatgaaatctttgttgttctggtagaacttgacgtaggaggacagcttggcgctgacaaatatggtcaaaagatcatgaatc
agttctccttccaggaatttgacaggctttaaagcaagaaggtgatcaagcaaaaaggctttgggatctttcagggcccttacgatgcacttgtgagcgt
ccagccgagcctgggaggcgttatcatccgtgtaactgccgagcagctccaccatcaccttcgctgcttcgtcacttttcttgcagtcgacaagagcttc
ataaagaagcctgagcacaatgtgtttcttctctgtgctgaggttccagtcagaaatccatttgcgaaantgggtcgagatctgtggggaatgtagatga
atggttccgcaggtggcttgcaactttttttagaaacccagtaaaacaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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