XB-CLONE-3916821
Clone Name: IMAGE:7092129
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Gene:
krt7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654556 [+]
taattaatcccccccccccccgactggatctgtctctcagaccatcagcagcagccttgcacctctcctgcactctcctgcactactactccaacctctc
actgagcaccatggctttctccagcagcagacaaaccagcttcagtacccgctccgcagtgcccgctgccagcttcagccaaatgcgcatcagctccact
cgctcctccagcggaggcgcagggctcggccgggcagggggaagcttcagcagctccagtctgtccaacatcggtggcggcgtcagaaagagcgtctctt
acggcgttagcagcggccgctccggtggcgcaggattcggtggcgctgggttcggtggcgcaggattcggtggcgcaggattcggtggcgctgggttcgg
tggtggagtagcttattccggacctgggatccaggaggtgacaatcaaccagagcctgcttgcccctctgaacctagagattgatcccaccattcagcat
gtcagacaggaggagagagaacagatcaagaccctgaacaacaagtttgcgtctttcattgataaggtcaggttcctggaacaacaaaacaaggttctgg
aaaccaaatgggaactccttcagaaccaaaaatccgccaagccagcaacgttggccccttgtttgaagcttacttgccaacctgaaaagcacctggatgg
ttgaatg
3' EST: CN319372 [+]ttttttttttttttagattgaaggaatttttattggagttgcttgtgtttctcaccagacataaaatccctcccacagaattcagatttccaccacaaac
acagaccatatctaaaagagtggtaactaaaacagaattgttccatatactgggtgttttacaactgcaaacaaacattaaggaatcactgttctttgtt
ttcccatctaagctgccagatttacatcagagacttactagaatttgttttacgttatatgttagcaccgtatatctcttttgttacctatcaagcctct
tctatgggaaaacaaaacaaacacaaagatatctgcccagaaatactgctaattttttatgtatttgttttaatacaggcatgagatttccattgtgaac
tgagcaatacaacttgcagcggtcgatactgtaaaaacgtgcccagacttatggcgaataggacacctagattcagcatacgattagagccagtcccact
ggtagaagggggagcagtgaaggagaaagatgcaagtaggcacttgtagacaggaatagggaactgctttcaagatttctgcaaacagccatatcctcca
gataataacaaacaacaaaatgcgggtgctagactacacacttggaaaggtgccaccaagatataggtatagggggtataacccactaagttaataccct
taccaacaccaacacattgtaaacagagcctgtttgaaattatttgaaggctatggctaatgctccctacaacaatacacaggggggaaacctttttttc
tgctgctggcacatctttattgtcttaaacctttcggccgggaaggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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