XB-CLONE-3916671
Clone Name: IMAGE:7092132
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Gene:
ccnb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654338 [+]
taattaatcccccccccccccagaggagttttactgcagtttgtgcaagacaagaggttgtggatttcccctatgatggcactgattatgactcggagta
tgcggctcaatgtggagaatgtggaaccaggcatggctggaaggaaagccgctgtgtccaagcctgtgctgaggcctaggaatgcactgggtgatattgg
gaaccaaataagcaaggcaaaagttcccctaaagagagcaacaaaagcacttcgcaaaccccctgtagagaaatcagagaaactgattgtaccagatgag
aatgaccctaaggaagcacagcccgtgcctgattctcctaaccgaatggagacttctgtgtgtgtaatagaagagatacctcctgctttctccagtgccc
taattccaatgaaagatgtagatgcagaagattcagacaacccaatgctgtgcagtgactatgtgaaggacatctactgttatcttaggaacatggaggc
tagacaagcaatcaggccacactatctagatggtcaggagatcaatggaaacatgagggccatattggtagactggcttgtgcaggtccatctgaggtta
aactgctgcaggagaccatgtctatgacctagctattctggatcgtttctgcaggaaatcctgttcccagaactgttgcgctgggctgggggagccatgt
ta
3' EST: CN320137 [+]tttttttttttttttaacacacttaaaataatctttattaaaaggataaaatacagttaagagcattggtaaaaagtgttataaacaggagtcttatgat
gcagcttttgccaggctttccacaagctcagacttcagatggggcaggcagctgaccttcatctgtcgactgctgctgtatttgctttttacagacagga
actttgtgtgtcctccattgacctttacaatattctttgcgatgtgctgcatgactggcataagggaactctctttataagccatgtagtgctcaagaac
aggagtccattctccactattaagaatcttcattgccaaacagaaagctgctgccgccaactgggagggaggtacgtgcaccatgtcatagtctgtcata
accagctctattaggtatttagccagtgtgtgatgtacagagtccacctccccaattttagatgctcttctcaagaaatgcagtggcagtggcctcccaa
tgtcaaatttgagaactctaaggatttgcatttccatgtttctgatttgggactttgtgtaagtgtggtcggtgacaaaggcaaagtcaccaattgttgg
acaatagatctcttcatatttgcatgcaatgaacattgcgctcacaccagccagctgcaacagtttcttgagaacaggatttttcctgcagaaaacgatc
cagaatagctatggtcatagacatggtctccggcagcagtttaaacctcagatgggacctgccccagcccagtctaccaaaaatggccccccatgtttcc
attgaatctcttgaaccatcctaaaatagggtggggcccggattggccttgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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