XB-CLONE-3916661
Clone Name: IMAGE:7091170
|
Gene:
sys1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK653697
[+]
attaatccccccccccccccgattggcattccggccttgcaccggaagtgtcgtcatccctaagcagctgtgatggcgaaggctggagagtggagacgct
ggaactggtaatggagttggttcggaagctgtgtttgcggagcggcctggagagtagttacgatgcggattacaatacattacatttaaggaaatgctga
tattctgagaaaggcttgctcctgtgaacaaatttcacagtgtttgtgtgtacattaaagatggctggacaattccgaagctacatatgggatccagtcc
ttatcatctcccagattttattaatgcaattaatctactacagctgcttaggactttggattgcagtgctggacttggtcgttcactacagcccttccct
tgaccagatatttaattgtggaattttgggattctcatcagttcccgggcgtgtctctatgatggcttttatcctgaattcgttgacatgtgctctgggt
ctattgtactttatccgcagaggaaagcaatgcttggactttactgtaacagtccacctgttccacctgtttggttgttggatttatacctcccagctcc
ccagttcattcacctggtggcttcttaaacgtgtctgcattgcttttatggcagtgatnagggagtatctgtgcatgcgaactgaactccgcgagattct
c
3' EST: CN320266
[+]tttttttttttttttcccatcctgattccgctttatttaatttttttaaaatccctgcaaattaggtgtgcattggctcatctatatttctgtcactgcc
agatatgaaacatgtcttagttcaataaattagcttaaacagaaatatattaatcctttaaggaaatatatagaccaaaatgaatatttctgtaaatggg
tggaaaaatataagaggcatacagaaacaaagtctgtaactgtgtgtgcaaagtaagcagggaacactcaggaacctgtgtcgtactgacatgccctttg
ggcagctgccattagcaaccctccctgcacatctagagctaaaaaactccagacaccagtttaaaaagccgacgatgtcaaccaatggaaaaaaatgtaa
gaatactgaagtactgaagattttggagaggtatgctgtaaacgtccagtgggttaaaaagtctacacattggatttgggtgctgaattaagaggaatct
cgcggagttcagttcgcatgcacagatactcccctatcactgccattaaagcaatgcagacaacgttaagaagccaccaggtgaatgaactggggagctg
ggaggtataaatccaacaaccaaacaggtggaacaggtggactgttacagtaaagtccaagcattgctttcctctgcggataaagtacaatagacccaga
gcacatgtcaacgaattcanggataaagccatcatagagacacgcccgggaactgatgagatcccaaaattcaacaataaatactgggtctaggggaagg
ctgtagtgaacgaacaattccacccctgcatccaaatccccaaacgcgcgttagtaaaata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: