XB-CLONE-3916659
Clone Name: IMAGE:7091245
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Gene:
rpl9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653589 [+]
taattaatcccccccccccccgcctttcctctgtcagctgcgagaatgaagaccattctcagcaaccagattgttgacatcccagagaatgttgacatct
ctctgaagggccgtacagttactgtaaaggggcccagaggtgtcctgcggaaaaactttaaccacatcaatgttgagctgtgtcttctgggcaagaagaa
gaggaggctccgggttgacaaatggtggggtaacagaaaagaactggccacagtgcgcaccatctgcagtcatgtgcaaaacatggtgaaaggagtcaca
ctgggctttcgttacaaaatgagatctgtgtatgctcactttcccatcaacgttgtcattcaggagaatggatctcttgtggaaataagaaacttcttgg
gtgaaaagtatatccgcagggttcgcatgagatcaggtgttgcatgcgccttatcccaagcccagaaagatgagctgattcttgaaggcaatgacattga
acttgtatcaaactctgctgccttgatccagcaagccaccacagtaaagaataaggatatcagaaaattcttggatggtatctacgtatccgaaaagggt
acagtacagcaggttgaagaataaaagcctgcaaaagtctggttcctgtgctggtccaaaataaagtccctttactaaaaaaaaaaaaaaggacnactag
ttctaaacggccgccccgcggggacttggccggg
3' EST: CN320267 [+]ttttttttttttttagataaagggactttatttttggaacagcacaggaaccagacttttgcaggcttttattcttcaacctgctgtactgtaccctttt
cggatacgtagataccatccaagaattttctgatatccttattctttactggggtggcttgctggatcaaggcagcagaatttgatacaagttcaatgtc
attgccttcaagaatcagctcatctttctgggcttgggataaggggcatgcaacacctgatctcatgccaaccctggggaaatacttttcacccaagaag
tttcttatttccacaagaaatccattctcctgaatgaaaaccttgatgggaaagtgagcatacacagatttcattttgtaacgaaagcccagtgggactc
ccttccccatggtttggacatgactgccgaaggggcgcactggggcccgttcctttctggttccccaccatttggcaaccccgaaccccctcctcctcct
ggccaaaagaaaccactcaacattgatgtggttaaagtttttccgcaagagacccctgggcccctttacaggaactggaccggccctcacaaaaaagtcc
acattctccgggatggccaccatccgggtgctgagaatgggcctccttctcgcacccgaacaaagagaaggcggggggggggggagaaatttacaccgag
tgaaaaaaaaaaatatcttggtttcgggccccgaaaaaaaccaggtgggtttggggcccgggggtgggcacccgtgggaaaaaaaagaaaaagggaaatt
ttttttcttttgttcccccctaattccttttgggattaaaaaataaggggttatttttttaattttttgaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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