XB-CLONE-3916613
Clone Name: IMAGE:7092010
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Gene:
sugp2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654575
[+]
taattaatccccccccccccctagaaaagccagtgttttggttcttgtttgataaggacagcagtgaatataaatattatcgtcaaaagcttgcacagtt
ccagaagtctaaaggtgaaacacaggaggccaaaattcaaacaaagaagctaaagaaagcccctgcaaagaccacaaatgaagcaatcagagccatgcta
tatgcaaaaaaagcgctggcagtaaggaaaagaataaataaaagggttgcatattcaagaaagcaaagactaaggaaggctagcgtcaagccctctacga
gcgagcctgtaaaacttgtaccaaaatctgagcccaaagaggaggtgtctgaagcaaagccagagattgaagagctgaagaaagctgaaagcaaaccaat
cacagacactacagaaactaccattgaaccaccaaaggtgaaagaggagaaggaagaggaggtaacagaatgtgtaaagcagaaatcctcatctgatcag
gaggagcttgatgacaagaccaaggacacagcagtaaaacttgcacantttgtagcacagatgggtcctgaactggaacagtttagcatggaaaacagca
ttaataacccagaattctggttcttacgagaaaagacagtccagcctataattttatcagagtaagttgaggagttcaacttgc
3' EST: CN320613
[+]tttttttttttttttgctgcttaagatactttatttgagtggttgtgaacttacaacaagccattttcaattgtaaaaacttatgaattgctttcttcag
gaaacattagcaaggcatttgtcaaagcaactaaaacctggaaacccaccttgattctgaacctctacatcacgtagcatttctttttcaaaaactaaat
ctgctggaatacaatgcacaatacccaagttactaagactgacaattaaaaagtatataaaatgtgattaaaaaaaaaaaactggaaagtaacaacactt
ttcttttaaccccactaaacaccaaacagcaatagctaaatgtcatgcaaggaggatgcctttcatatggctcaaaaataaaaaagggtaataattaaac
cctgaacatctactgtaggaatttttttcatggattgctgttttcttcaggaatcctgcaagactactcatttggttattttttgcctgtacatttgcat
cattctttgacggaaagcatcaaagttggtctctgaagactcaccactttccacacctaagccctctcctgaagagattgaacccactttaacaggattt
ttgatccctgacccactggtgcctagcccttgtcctttcctccatcccatcttgctcagcatttgggaaccccacttcccttctgttaacttcttgttgg
gcaactctagaataaatggtttcttgtttccgttttaaccgcggccccggggccttttcaattcaaggcaattggaatatggccttgggggccttcccca
aaaaagaatcctttgggggggagcagtcccaactttttaattttgtaaaacacctttcccccccttaaggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: