XB-CLONE-3916485
Clone Name: IMAGE:7091313
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Gene:
ilf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653929
[+]
taattaatctcccccccccccagtgtggctgtatcgagggccgagagggtgtccattgtgcgggagtgtgaggaacgaagatctgaaacatgaggggtga
acgaggacgtggacgtggccgctttggctccagagtgggacctaacacaggtttcagacccttcgtccctcacattccctttgatttccacgtgtgtgaa
atggccttcccgcgtgtgaaacccgcccccgacgatagctcattcagcgagtcactgctcaaacgaaaccaagatctggcccccagcacagccgagcagg
cctcaatcctttctttggtcaccaaaatcaacaacgttatcgacaatttgatcgtagccccgggaaactttgaagtgcaaattgaggaggtgcgacaggt
gggctcctacaagaaagggaccatgagcacaggccataatgttgcagacttggtcgtcatcctaaagatccttcccacactggaagccgtttctgctttg
ggcattaaggtggtcgagaccctaaggacgcaggatccagctgaagttctcacaatgctgacaaacgagactggctttgagatcagctcagcagacgcca
cggtgaagatccttatcacgactgtgccgcccaatctgcgcaagctggaccccgagttgcatttggacatcaaagtctgcaaagcgcgctggcgccctca
ngacgcacggtggttca
3' EST: CN321285
[+]tttttttttttttttggggtgtcgaaataaaaatttattgctggcaaaaaaaaaaaaaatacaatgtatccctacagtataaaacaagtcacatgacaag
ctaagaacgaatgaaaataaaacaggaactcgtggcatttggtccacaaacagacccaatgtgttcggcgccggcagagggggatccagaccaactgaaa
tctttaaataagtatcactcctgtgtctccatgctctcctcctcttccgccccctctgggatatcctggttctcatcctcctctccctccttcctctcgg
ggggcttctcataggccttctctgaaggggtcacgatgactccgtcccaggtagacatttccgtggcaagagcgctggcatctccctccaggcccaatat
cttccggaagcccccgtgggagagaatacggaccaacgtctgggcagtgtagcaaaccatgtcctgctgctccagagtcatgactgtatgaaccctaaag
ttgccactttcacaggggtcagtgatgccaactgagccggggaggaaaaggccggcggcgagcatctgtaggcagcgtttgtaggacacattgagtgcca
gcggctgcctgggtggggttgttcatgacggcataatgccccaagaggtccaggatccagggagtcagaggctcaaaatccagaaaccgactcctncagt
ctttcacaaccggattaaaacttcacttgtagaatgggaggcgttctcctcaaccnaccgtgcgtgcctgatggcggccagcgcgctcgtccagaacttt
gatgttcaaatgcaactccgggttccaccttggcccaaattggggcggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: