XB-CLONE-3916467
Clone Name: IMAGE:7091395
|
Gene:
ckmt1b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK653626
[+]
taattaatccccccccccccccaggtcactgcatctgcagagaagccaccatccaggacaagtcagcacacacagagcttcaggatccccaaaatggcca
gtactttcaccagaatcctgtcctcaaggaaatcagctggactcctggcaatggttggagcaggttctgtagcagctggatacctaatgaccagagaagc
aatcagtgccgacaccagcaataggaggctttacccagcaagtgcagagtatcctgatctgcgcaagcacaacaactgtatggcaagcaatcttactcct
gccatctacaccaaactctgcgacaagaagacacctgcaggtttcacggtggaccaatgcatccagactggggtggataacccagggcatccctttatta
agacagtagggatggtagcaggagatgaagaatgctatgaggtgtttgcagatctgtttgaccctgttataaaggaacgccacaatggctatgacccacg
aaccatgaagcaccccacagacttagatgcaagcaagatcaaaggtggcttttttgacgaacgctatgtgctttcctctcgtgtccgtactggccgaagc
atccgcggtctgagcctgcccccagcttgcaccgagcagagagaagggaagtggaaaggtgacagcggatgctcttatggactcagggagattatctgga
acta
3' EST: CN321477
[+]tttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttcccccccgggg
ggtttttttaaaaaggaaaaggggaaaggtttaaaaaaaaaaagggttttaaaaaaatccccccccccccccacccgggggaaaaaatttggggggcggg
ggaaaaaaaacccccccccccaacccgggggggggaaccccggggttttttttttccccccccccctcccaaaaagggggccgggggccaaaattttggg
gtttcaatttttgtttaaaaggggccccgggggaaaaaaagggaaaatccccccccttccccaaaccccccccccccccaaaaggggaaagggcccccca
aaaaacccccggggcccccccccccccaaaatttcccaaaaaacccaaggaaaaaaaaaaaaagggggcccccaaaagggagggggccaccccccccggg
ccccccttctaaaaagggggggtttccctaatttttccaaaaaaacccccccctcccaaaaaggagaattaaatggcccccccccccccccgggcggccc
cccatgggaggggaaaaaaaaagaaaaaaccccccccccccccccccaagaaccccccccccctttttttagtaattcccccccccccccccggggcccc
aaaaaaaaccccaaaaaacccctcttaatttcccccccttttttaaaaaaaaaaacccggggggggccccctctaaaaaccacaaaaaaagaagagggcc
ccttttgtggtgttgcccccttccccccccccccccgggccgcccccccccccccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: