XB-CLONE-3916461
Clone Name: IMAGE:7091230
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Gene:
idh2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653712 [+]
taattaatcccccccccccccacagattttgtggtcgatcaggctggaactttcaagttagtctttgctccggcaaatggaactgccacaaaggaatggg
aagtgttcaactttcctgctggaggtgtcggcatgggcatgtacaacacagatgagtccatctctgggtttgcacacagctgctttcagtattccataca
gaaaaagtggcccttgtatttgagcacaaagaacactattttgaaggcttatgatggcaggtttaaggacatcttccaggacatctttgagaagcattac
aagccagaatttgacaaactgaagatttggtatgaacacagattgattgatgacatggttgcacaggttctgaaatctaccggaggcttcgtttgggctt
gcaagaactatgatggagatgttcagtcagatattcttgctcaaggctttggttcacttggtttaatgacctcggtcctggtttgcccaggtggaaagac
tattgaagctgaagcagctcatggaacagtcactcgccattacagggagcatcaaaagggaaggccaacaagcactaaccctattgccagtatttttgca
tggacaagaggcttggaacacagaggcaagctagatggaaacagggactaattaacttctctttacacttgaaaagtctgtgtgan
3' EST: CN321505 [+]tttttttttttttttcaagatcttccaatatttattttctgtacacggcaccaggtaagaggttgctgacaaaataaaggtgtattaaaaatttaggtcc
agcactaaacacaggcaactctaaggaccagtcccattcagttgcggtactttagggtgtttaaaaattcgaccagcatttaatgggttaaaaaatttgt
tccagagccaagctattttggcaattcctattttgaatggttaaaaatgaggtaaaaaagttaacaggctgttaagctacagtatgttaattcttcccaa
gagctttgtcgaggttgttcttaattgcatccaaaaagtctgtcgtgttcacataatgttcattaagacgaacgttgctcatgcctccatgaatacagcc
agccaagtcttttgtcatgatgccactttctacagtctccacacagaccttttcaagtgttaaagagaagttaattaggtcctggtttccatctagcttg
cctctgtgttccaagcctcttgtccatgcaaaaatactggcaatagggttagtgcttgttggccttcccttttgatgctccctgtaatggcgagtgactg
ttccatgagctgcttcagcttcaatagtctttccacctgggcaaaccaagaccgaggtcattaaaccaagtgaaaccaagcctttgagcaagaatatctg
actgaacatctccctcataagtcttgccagcccaaacaaagcctccggtaaaattcagaaactggtccaacatggtaatcaatcaatctttggttcaatc
caaatccttctatttttccaaattttcgggcctgttaaatgct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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