XB-CLONE-3916459
Clone Name: IMAGE:7091959
|
Gene:
cnfn.1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK653800 [+]
ttaattaatccccccccccccccccacacancatccancaacctcatctcccatcnactgancaatgtctgcagcacaagtcacaatgtcttatccaatc
ggcgctcagccccagggtgtgcaaggttacgtcaccagtaacagcagccagtggaattctgatgtctttgactgctgcgaagacatgggcatctgtctat
gtggaacgttcgttccctgcattttggcttgtaaagtgtctcaggatttcggggagtgctgctgcctgccatgtctgttcggctctgtccttgccgtgag
aacaggaattagagagcgctaccacattgagggttctatctgtaatgactgggtgtgcctctcattctgtggccaatgcactctctgccaaatggctcgg
gaactaaaggccagaaactgagaagactagaaggctggagaagggggtatggacaggacaatgtggacttttgtctgaaggacccaacaacttcctattg
gtttatagaatgaaacagctactggaccctaacttacatgcatcagtgcacattattattcccatttatgtatatacacgtgtatctactggaactggat
atcaaaaaaagtaacagaagattgaatggcaattttaaaaaaggggtaaaaaggtattaactggaaagggaatgaaaaattctttcttccctctggctat
aaaatgcccccttgtttgtttgtttt
3' EST: CN321516 [+]tttttttttttttttcagtaaaaaaatatttattaatattttaattcttaaacaagcaaaatcatggtaatgttgcagaatgtggctcaggtacacagag
tgctgagatcctgtagttgcagctacagtaagtgtgggttgtgccccccacccactgcttaactgcatctactatgaatgtcactcagccattggcttga
gatctctcctgtttatatccccgatgttctcaaaaactgaccccccaaccactgttacatatttagcgtatattacaactaaattcccaggggtttcgtt
atagtgagggtgctgcaccaaattaggcttgctgatattaattctgagctatggccactgttttctttcatataatcacaattacaatgaatggtctgga
agtggaggagaagaagatcacaaggccacattttctgtctctgtcacattcaaaggtcaccaaaaatgattgcccttgtctgttggtctgaaaacatggg
atgtactgataatgaattaatgtagaaatataataccaatataatattgtctactgctgtcacgctgttcatgggctatggttatgttggaaagttcagg
tcctcttcagggaggctctaccagctctgttccagtacaactcccatcatatcagccaataccagatgaggtacaaagattttggacattagaaaaggta
taagccacagccttgtgtaatatataataaacaacgtacaaaacaaacaaacagggtggcatctattagccagagagaagaaagaagatctccatgtact
cctcagttaatacccttttgtacccctttttaaaatttgccacttccccctccgggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: