XB-CLONE-3916413
Clone Name: IMAGE:7091227
|
Gene:
fkbp2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CK653913 [+]
taattaatccccccccccccccgaagctaggagcctgcacatagcagagagttgcgggacctgcctgtgttttgctgcaacttgtcagagccctacctgc
cataaatatgaattttatgtcattcacacctctgtggtgtttccttgtcctgttcctgctgccttctgtctcagccagtgagggcaggaagaaactacag
atcggagtgaagaagcgggtggaaaattgccttgttaaatcccggaaaggagacacactacacatgcattatacagggaagctggaggacggaacagaat
ttgacagcagcattcctaggaatcaagcgtttaccttcactctgggtaccggacaagtcataaagggctgggaccaaggattacttggaatgtgtgaggg
agagaagaggaagctggtaataccttcagaattgggttatggagataggggagcacccccgaaaattccaggaggcgccactctgatctttgaagtggag
ttagtgaagatagaacgtcgatctgatttataaatatgaggccatgccagccttctgtttagggaccagtatttgtgctgtgaggggagacagtgtgtca
ccttgtacccaataaacacacacacacactacttctatggtttcattaataaccatagagttacattcccgggctcacccaaagactcatgttcctgaat
tatggttngctacagtacn
3' EST: CN321847 [+]tttttttttttttttggtagaaaataaatggtgtttttgaacaaatttgaagcaaaatgacataacattaagcaaccttgtgtttaggctaaatagtatc
tcaaaggaagggaaaacatgcaagtgtttattgaaattgttatcccatcaaactctttgctccggtacctgtagcaaaccattaattcaggaacatgagt
ctttgggatgaagccgggaatgtaactctaatggttattaatgaaaccatagaagtagtgtgtgtgtgtgtttattgggtacaaggtgacacactgtctc
ccctcacagcacaaatactggtccctaaacagaaggctggcatggcctcatatttataaatcagatcgacgttctatcttcactaactccacttcaaaga
tcagagtggcgcctcctggaattttcgggggtgctcccctatctccataacccaattctgaaggtattaccagcttcctcttctctccctcacacattcc
aagtaatccttggtcccagccctttatgacttgtccggtacccagagtgaaggtaaacgcttgattcctaggaatgctgctgtcaaattctgttccgtcc
tccagcttccctgtataatgcatgtgtagtgtgtctccttttccggggattnacaaggcaatttttccacccgcttcctcactcgaatctgtagtttctt
cctgccctcacctggctgagacaggaaggcagcaagaacaggacaaggaaaacccacagaagtgtggaatgacataaaattcatattttttgcaggttag
ggctctaaacaatttgcaacaaaaacaaaggcagggtcccgaaccccctgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: