XB-CLONE-3916393
Clone Name: IMAGE:7091026
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Gene:
sec23a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653947 [+]
taattaatccccccccccccccccggactgactgagagagctagtggagcggagagtaagaggacaggggtgtcttgcgatgaccacctttctggaattt
attcagcaaaatgaagacagagatggggttcgcttcagctggaatgtgtggccatcaagccgcctggaagccacaaggatggtggttcctgtagctgctc
tcttgacaccgctaaaagagcgaccagacttgccccctatacagtatgaacctgttttgtgcagcaggactacatgcagagctgtactcaatcctttatg
tcaagtggattatcgagcaaaactttgggcatgcaacttctgttaccaacgaaatcagtttccccctacatatgcaggcatctcagaaatgaatcaacct
gcagagcttctgcctcagttttctagtattgagtatgtggttcagcgtggacctcagatgccactgatctttctgtatgttgtggacacttgcatggaag
atgaagatcttcaagctcttaaggagtccatgcaaatgtccctcagtctgctgcctcctacagcactcgtggggctgataacatttggacggatggttca
cgtacatgaactgggatgtgaaggcatctcgaagagctatgtttttagaggaaataaagaccttactggaaagcaatccaggaaatgt
3' EST: CN321976 [+]tgcagctctcctagaaggtcagtgagattcatgtcgatgttctgcactggttgtaaaaatctgttggatggagggggttgttgaacttgtggcccacggc
cttgttgggctgctggagattttgtaagagataacatttcctggatttgctttccagtaaggtctttatttcctctaaaaacatagctcttcgagatgcc
ttcacatcccagttcatgtacgtgaaccatccgtccaaatgttatcagccccacgagtgctgtaggaggcagcagactgagggacatttgcatggactcc
ttaagagcttgaagatcttcatcttccatgcaagtgtccacaacatacagaaagatcagtggcatctgaggtccacgctgaaccacatactcaatactag
aaaactgaggcagaagctctgcaggttgattcatttctgagatgcctgcatatgtagggggaaactgatttcgttggtaacagaagttgcatgcccaaag
ttttgctcgataatccacttgacataaaggattgagtacagctctgcatgtagtcctgctgcacaaaacaggttcatactgtatagggggcaagtctggt
cgctcttttagcggtgtcaagagagcagctacaggaaccaccatccttgtggcttccaggcggcttgatggccacacattccagctgaagcgaaccccat
ctctgtcttcattttgctgaataaattccagaaaggtggtcatcgccagacacccctgtcctcttactctcgctcactactcccatcatcccgggggggg
ggggggggggggggggggggagtatttttttctcgcgggtcccaagaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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