XB-CLONE-3916377
Clone Name: IMAGE:7091970
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Gene:
fxr1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654568
[+]
taattaatccccccccccccccgcccatcggcctttgcgtttccaacatggaggacctggcggtggaagtgcgcggctctaacggagcttactacaaggg
atttgtcaaagatgtacatgaagattccctcacagttgtttttgaaaacaactggcaaccagagcgtcaggtgcctttccatgaggtccggatgcctcct
ctcccagatattaaaaaggaaattactgaaggagatgaggtagaggtgtactcccgagccaatgaccaagaaccttgtgggtggtggttagcaaaagtac
gaatgatgaaaggagagttttatgttattgaatatgctgcttgtgatgctacctacaatgaaattgtaacatttgaacggctgcgacctggaaaccagaa
taaaagtgtaacaaagagctccttcttcaaatgtacagtggatgttcctgaagatttaagagaaccgtgttctaatgaaaacgtgcataaggaattcaag
aaagctgtaggagcctgccgagtttattttcatgcagaaactaatcagctgataatactgtctgcatgtgaatcaactgtaaagagagtaaccatattga
gcgacatgcatttgagaagtattcgcacccaactgatgcttatgtcaagaaatgaagagcccccaaacatttagatgtacaaacan
3' EST: CN322006
[+]tttttttttttttttcctctgctagaagaaggtctgaagcccataccaatctggcgaagttgctcatcgatctgtagcctttccattctaagttgctcta
cctcctttaagtatgcaatgtgatattccagaagaacctgtacattaccaatgctctctttggttccaacaaaaacaaatggaaccataccatcttcacg
gggtaactttgtttcgttgtctccctcgattctgactcgaacgactccagatttatcaacaatttcttgaatgacttttccatttttgccaattactttt
cctacaagatttctaggtacttgaatgaaatcttccacaaactccaagtaacttctggcttttttaactgcttctgcactctccccataaattcgaaatg
tgccactatcttcatctaactcaatagcagtgatacctggaaccttccgagcttgttggatattacttccgtgtgtccctattgccagacccattaaatc
ttcccggacaacaaattcttcatgaaatgctgctgcaagctgttttgtacattctaaatgtttttgtggcctcttcattttctgacataagcatcatttt
ggtgcgaaatattctcaaatgcatgttcgctcatatggttactcttctttaagttgaattccatgcaaaaagtatttataagctgattattttctgctgg
ataataaacttcgaaggcttcctaaatttttcttgatttctttagccagtttttcatttaaaaacggttcttttaaatttttaggaaaatccctgtgaaa
tttgaaaaaaaggaaccttttttttaacattttttttttggtttttccgggtctcccccccctttttaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: