XB-CLONE-3916295
Clone Name: IMAGE:7091223
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Gene:
uqcc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653911
[+]
taattaatcccccccccccccatgtaaaggcgtgtgaccggactgaaggtttcaatgcgtggctactgtcctaggatccgaagtattctcaaaagtcgac
actaatttgaaagttcaaaggcaacagtaccatcatccagctttcatatccataaaaagtcacagaaaataaggaaatggcgtctttacggtaccgtcgt
tttttgaaattatgtgaggaatggccagtggatgagacaagagttggccgggacttaggtgcatttattagacaacgggtagcacaagcctttcgtgagg
gcgagagcacacaggtggatgatccagatgcctgtgacgaaatgtatgctagtttaaacagaatgaatacaaactactacagagagaagtatcctcgact
ccaagatacaagcttcactgaggtcactgcagaagaatataaaatggttctggcatctgacaacctaaagcagatggaagagatgaaaaagggaatgtgg
aagagacttcgagataagtttaatgtttaaccatcagaggagaatagctgaaaagaacaaaccaacttcttccttatgcattctaccacatataaataat
ctaccagtacacagggcactttcctaggtactttaaagttgactcaagttattgaatggacatggagtttccttgagtcacgctgttgttgtgttatctt
aattttgataatcacttgcctgacatgtacagtaaatc
3' EST: CN322440
[+]tttttttttttttttgtaaacattaaaatttattggcaaaccactgaagacatttccagatgtaacttgtacatgtcagtgcaagtgaattatcaaattt
aagataaacacaccaacagcgtgactcaaggaaactccatgtaccattcaataacttgagtcaaactttaaagtacctaggaaagtgccctgtgtactgg
tagattatttatatgtggtagaatgcataaggaagaagttggtttgttcttttcagctattctcctctgatggtttaacattaaacttatctcgaagtct
cttccacattccctttttcatctcttccatctgctttaggttgtcagatgccagaaccattttatattcttctgcagtgacctcagtgaagcttgtatct
tggagtcgaggatacttctctctgtagtagtttgtattcattctgtttaaactagcatacatttcgtcacaggcatctggatcatccacctgtgtgctct
cgccctcacgaaaggcttgtgctacccgttgtctaataaatgcacctaagtcccggccaactcttgtctcatccactggccattcctcacataatttcaa
aaaacgacggtaccgtaaagacgccatttccttattttctgtgactttttatggatatgaaagctggatgatggtactgttgcctttgaacttcaaatta
gtgtcgacttttgaaaatacttcggatcctaggacagtagcacgcattgaacctctctgcgccccccctttttgtgtggggggggggggggggggggggg
ttgtttttttctctggagggcaaaaaaaaaaaaattttcttcgtttttcggggggccggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: