XB-CLONE-3916227
Clone Name: IMAGE:7092105
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Gene:
ncl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653829 [+]
taattaatcccccccccccccagtcggttccttgttgcaggggcatcgtgtcgtcaggatcctagacacgtgaattaccattctctcccaaacacaacac
cggccaccatggtgaaattagctaagggtgcaaagacacaggctaagcccaagaaggcggcaccaccacccccaaaagatatggaagacagtgaggaaga
ggaggacatggaagaagaggacagcagtgatgaggagatggaactgcctgtaaagaagacaccagccaaaaaggcagccactccagccaaagcaacatct
gcaaaggcagccactccagctaaagctacagtcacaccaggaaagaagggagcaactccagcaggagccaagaatggaaagcaggcaaagaaacaagaga
gtgaagatgaggaagatgactctgatgacgaagctgaagacccgacgcctattacaaacaaaccagttgctaagaaagctgtagcaaaaaaagaggaatc
tgaggatgatgaagaggatgatgaagatgagtctgaggaggaaaagccagtttccaagaagccccttgccaagaaccctccagctaaagttacacctgca
aagaaacctactgggaagaacaggaatctgaagatgaagagatgagtctgaagaagaaagccctgtgccaagaaccctctgcaagaaactgcagcaaaaa
caagatctgatgagagaactga
3' EST: CN322825 [+]tttttttttttttttgtcaaaagccatagccttctcaatcttcacctctgaaccaagtatctttttcccactcagctttaaagcctgctcaacctcttct
tcagatgagaaatccacataaccaaacttctttgagtttccaatccttacatcctgcacagttaggttcttcttggaaaagaactccctcagagcatctt
taagctcatcaaaatcttttgaggcgtttaagttgccaataaaaatggacaatccttctgatgtgtctgtctttgctttttttgcctcaggaatattttt
agtctttggcatttcttttttgcgttttgcagacacttgttgttcttcgtcatcctcatcctcctcatcatcatcgtcgtcatcctcgtcgtcgtcctca
tcatcatcatcatcgtcgtcatcctcatctgctttggcagggacagttttcttcccctttagagctggtgcaacttccataggctcgtcttcatcatctt
cagattcttcatcatcagattcttgttttttgcctgcaggtttctttgcaggagggttcttggcaacaggcttttcttcctcagactcatcttcttcatc
ttcagattcctgtttcttcccagtaggtttctttgcaggtgtaactttagctggaggggtcttgggcaggggcttcttggnaactggcttttcctcctca
gactcatcttcatcatcctcttcatcatcctcagatttctctttttttgctacagctttcttagcaaactggtttgtttgtaatagggcgtcggggtctt
cagcttccgtcatcaaagtcatccttcctcctctttcccctcctctgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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