XB-CLONE-3916225
Clone Name: IMAGE:7091249
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Gene:
stt3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653591
[+]
taattaatcccccccccccccgagcagagggagaggagcggctgccaggtttctagtgttttctgtcccttccctttatgtaaaatgactaaattaggat
ttttgcgtctgtcctatgagaagcaggacactctcctcaagctcctcatcctgtctatggcagcaattctctccttctctaccagattgttctcggtttt
gagatttgaaagtgtcattcatgagtttgatccgtatttcaattatcgaacaacaaggtttcttgccgaggaaggattttataatttccataactggttt
gatgatcgagcatggtatccattgggaagaattataggaggaacaatctatccaggtctgatgataacctctgcagcattctaccatattctacactttt
tccacatcactattgacatccgaaatgtttgtgtattccttgctccactcttctcgtctttcaccaccatagtcacttaccatctgacaaaagagctgaa
agatgctggtgcaggccttttggcagcagcaatgatagcagtggtgcctggctatatatcacgttctgtggctggatcctatgataatgaaggtattgcc
attttctgtatgcttctaacttactacatgtggattaaggcagtaaagactggttcatctattgggcagcatgtgcgctctagcatatttctattggctc
ctcgtgggaggatactgttctgat
3' EST: CN322826
[+]ttttttttttttttttgtttccccatctttaattgaccacggtaaagtcagaacaacaaaaaaatcattttggtagcattcatattcataaaaagcattc
cacccaacatccacagcaaccagtgtgacttccacagccaaataagttctttctttccgtcaataaaaacagtataaaaaaaaacttctggttcacaggt
gaaagctctttcaatgctgtttgtattaaagctgaaaggaaaaaaaattatggtcctgataaccctcgattatccaaatacttttctttacacattctaa
ccagccaatgctatgtggcgtaagcttcctctaaaaaaaaaagccccaaatccttattgcccatttccgcgtttctcacatcgactaaacaggaaggcct
atagttaccgtggactattttaggaaacaacatatatacatattcgcataagaacatcttaaaccaaccttgccaaccctttctgtaaaaacttttatcc
cccagttgactgaagtcctcctgtttcttcgaaggtgcaacaaatccacctcaggtccccatataataccatctgtaacaactctagttacattgacctt
atttttttcttccagtggggctaacaattttgcccttttgccccaagggccctggttcctattcataaatacaccgactttcaccaaatgaagcccttat
tccctgtcatccattgtaacagggaaaggcacaccaaaaaaaaaaaaaactagagctagttgtcttccaccgacgagccccttaccccctaaaccaccca
tacccccaaccccccttttttaaaaaagggccggaaagtcttttttttttgaaaaatccccccctctgttgcccctcggggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: