XB-CLONE-3916175
Clone Name: IMAGE:7091940
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Gene:
vdac3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654301 [+]
taattaatcccccccccccccgcagagaccttcagccggggacagtaaacatggccgtgccacctacatacgcagacctgggcaaagccgcccgagatgt
ttttaataaaggatatggatttggcctggtgaagctggatctcaagaccaagtcctccagtggagtggagttcacaagcagcggctcatccaacactgac
acagggaaagcatcaggcaacctagagacaaaatacaagatgaaagaggctggagtcaccttcacccagaaatggaacacagacaacaccctggggactg
aagtggccattgaagataagttggcaacaggtttgaagctctcagtggacactacatttgtgcccaatacgggtaagaagagcgctaagctaaagacctc
ctacaaaagagaccatgccaacgtgggcattgatatagacctggacttggctggtcccaccatatatggctggggggtgcttggctatcagggctggctg
gctggctatccagatggcattcgacaccgcntaatccagactggcccaaaactacttttgcttgggctatacagcaggaaacttccagcttcctacaaat
gtccatgatggcacagaatttggggggctcatggaccaaagggtaataaggatggggagacgtcatggagcttgcttggagggccgcagcaaaacactct
ttttggtttggggccattccaacgggctcgggacccttccctgcaa
3' EST: CN323115 [+]tttttttttttttttgacctttaaagaactgtatttaatgaaaacacttctaacaatgggggaatgcattaggatttttttttcctcattttgatacagc
agcatctctgggacagcgaccaatataacaaaaatgacacagaatagctaaggaataaccaagccccatgttgggggggtgagcacatattaggctatta
gtaaattaggccttctgatccaaaatgggtcagtgaaagagtggggcagacagtaagtgctagcgggggtgtaacaattactagggtggggatgatggta
agcacccgcctgggtggggaacaggaaaaagaaataataaactttattattaatcattatatacttactaaactattttattacatgctctctgtattgg
gggaagacttttggaccttgggatttcagtcgtcaatatgatctttttaaaaattattatattgatttctcctttttttttttattttatatgatgtatc
ttgtcctttccaattctctttactgttttctctttgcttcagcgcagaatactattattatttttttctttatagactattcattcgtgatcattgctta
tctggattttcgcctatatagttctacctcttccagactctgatcctcttctttagtacactttcttttcatgatgtcatcaacctttaataatcactaa
atcctttttgttcttttattattctcttttcccgtctttataccctattataatcaccatttttatggtcaaattacttttcagatcttctgttaatatt
ccacatctactttttctgtgtatattcataattatttcttttatttcaatatcattctctcctttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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