XB-CLONE-3916123
Clone Name: IMAGE:7091851
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Gene:
ran
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654504 [+]
taattaatcccccccccccccggacgcttctggaaggtgagattctaacccgtgggagatataggctgggccctgccgcataatccgcccgacatctcgg
ccatccctattcgcaaagcgtcccgaagctcttgtagcactcgctgattaatcgtgcggccgggcacggtagatttgagagggattctagtcggctgaca
aacataccatggcagcccaaggagaacctcaagtgcagtttaagcttgttctggttggggatggtggtaccggtaaaacaacctttgtgaaacgtcactt
gactggtgaatttgagaagaaatatgtagccacacttggtgttgaagttcatcccctggttttccacactaacagagggccaatcaagtttaacgtgtgg
gatacagctggtcaagaaaagtttggtggtctcagagatggatattatatccaggcccagtgtgccattatcatgtttgatgttacatcaagagtcacat
acaagaatgtaccaaactggcatagagatcttgtaagagtgtgtgaaaacatccccatagtcttgtgtggtaataaagtggacataaggacagaaaattt
aagccaaatcaattgcttcctaagaagaagaacctcagtactataaatttctgcaaaagaaataaaaatttgagaacccttctttggct
3' EST: CN323400 [+]ttacaacctctggtggggcaagggctggcatggctacaaactcaagattggggtcacctataagtttccttgcaagccaaaggaagggcttctcaaaatt
gtagttacttttagcagaaatatcatagtactgaagattcttcttcctatggaagacaattgatttggctttaacttttctgtcctttatgtccacttta
ttaccacacaagactattgggatgttttcacacactcttacaagatctctatgccagtttggtacattcttgtatgtgactcttgatgtaacatcaaaca
tgataatggcacactgggcctggatataatatccatctctgaaaccaccaaacttttcttgaccagctgtatcccacacgttaaacttgattggccctct
gttagtgtggaaaaccaggggatgaacttcaacaccaagtgtggctacatatttcttctcaaattcaccagtcaagtgacgtttcacaaaggttgtttta
ccggtaccaccatccccaaccagaacaagcttaaactgcacttgaggttctccttgggctgccatggtatgtttgtcagccgactagaatccctctcaaa
tctaccgtgcccggccgcacgattaatcagcgagtgctacaagagcttccggacgctttgcgaataaggatggtcgagatgtcgggcggattatgcggca
aggcccaagctatatctcccaccggttagatctcacttctaaaaaacgtcgggggggggggggggggaaaaataaaaccagtgggaaaaaaaaaaaaaac
ttgtgttttggggccgggaaaaaaccaagggtgcatttggaacg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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