XB-CLONE-3916087
Clone Name: IMAGE:7091842
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Gene:
tmem59
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654282 [+]
taattaatcccccccccccccccgagttactatatgtacgaagatggcggatacaagagggctactgctcgttctcctgctttgtgccagcgctagggca
acagagcccgctgggaatgtagactccctgctgggggacagcgcgtcttgtcacggtgtgtgtgaggcaacctaccccctgcatacctaccccgaggagg
aggagctgtttgcctgtcaaaggggctgtcgcctcttttctatctgccagtttgtggatgatggagaggacttgaacagaactaaagtagaatgtgaatc
agcttgcttggaagcttatccacaatccaccgagcaatttgcttgccacttgggctgccaaagtcagatgccttttgcagcaaagcgtcaggaagagctt
gctgatttagcccctcggattcacatcctttttccccttgcctttgtgggagctttcatgagagacatgatggattctgcacaaagtttcattaatccat
catggaccttttatgttcaggcagataatgggagaattattgttgtccagtcggaatctgaaatccagcatgatccagaaaactttttgccagaaaaggc
acaatctgccgaacgctttctaaacaaaatatccttagatcttgtgcctggtgaatctacagaaaatctggagaaaaaagacttggaaccgcgactggac
tacttgatttgaaaaagcaacact
3' EST: CN323552 [+]tttttttttttttttagtaacattttaatatggaaaaaaactgcattatacattagtcaccttgtaaagaatacaccctcaaattcagtcaccccaaatt
tgtgccataacacctaatggtctgtgatgatgcgactgctgaacaaatagccagtcgaagcttttcacatacattacttctttgtgtcagatatttctcc
acaactatcaccattaacaaccaataactaacacagtattaagtactctgtatgatttaaaaatcagtggaagtttctaactgattgaaagagttaccat
atttatgtagcagtctttcttattgcacaaatctggatatctctacctccttataaatgctactgcagcagtaacaacctgaaatacctaacttattagc
taaacaatgaatcctccccaatatagccccaaaggcttgttggatttaaacaaaaatatatttaatctatcttgtatggttcccaccaacaaatattgta
aaaagatttaaaaattagggaagtaattcaatcaaagaaatatgctaaatgcctttaataaggcaaaatctttcctaccaccaaagcaatctgcatcaca
ggaactacacttccagcaacaatcccataataaagatttccttttcccaaagattatggattggaaagatggcagcaacatcatttgatttctgttgtta
aagaaatgattcctgtgctgcatatggctgtacaaaaggtgacaataagacagaacaacagctgcctgccaggaattaaatggatgaccccattaggtcc
accctttgtccggcaaagttcctggctccttcccttttttttctgtttttccccggggaaaaaaaaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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