XB-CLONE-3915981
Clone Name: IMAGE:7091069
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Gene:
ran
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653873 [+]
taattaatcccccccccccccggctgctttacagacgcttctggaaggctgacaaacataccatggcagcccaaggagaacctcaagtgcagtttaagct
tgttctggttggggatggtggtaccggtaaaacaacctttgtgaaacgtcacttgactggtgaatttgagaagaaatatgtagccacacttggtgttgaa
gttcatcccctggttttccacactaacagagggccaatcaagtttaacgtgtgggatacagctggtcaagaaaagtttggtggtctcagagatggatatt
atatccaggcccagtgtgccattatcatgtttgatgttacatcaagagtcacatacaagaatgtaccaaactggcatagagatcttgtaagagtgtgtga
aaacatcccaatagtcttgtgtggtaataaagtggacataaaggacagaaaagttaaagccaaatcaattgtcttccataggaagaagaatcttcagtac
tatgatatttctgctaaaagtaactacaattttgagaagcccttcctttggcttgcaaggaaacttataggtgaccccaatcttgagtttgtagccatgc
cagcccttgccccaccagaggttgtaatggatccggcattggcagcacagtatgagcaagatttacagcatgctcanggcactgcactgccagatgaaga
tgatgatctgtga
3' EST: CN324160 [+]tttttttttttttttctttttttttttttttttatttgaaacataactttattatgatctaaacgaaaaaggtatgggaatgacagtaacaagattcacc
actgaattttgtgatgtgactgtagcagtcttaacttaagatttgaaacttgagggtagaatatttgcagtcccaaacagctaaagacgcaggcccaaaa
tatgaaggaattattttctgaactgccacattcactccgaagcccattcatctccttctgcatcccaaaaagattaagcacatgttctgctcagctatat
aatagagtggcaaacacgctgcaccacatcacaggacagttgcctataaaactagacttctgacgctgggctccagcttcattcccctcacagatcatca
tcttcatctggcagtgcagtggcctgagcatgctgtaaatcttgctcatactgtgctgccaatgccggatccattacaacctctggtggggcaagggctg
gcatggctacaaactcaagattggggtcacctataagtttccttgcaagccaaaggaagggcttctcaaaattgtagttacttttagcagaaatatcata
gtactgaagattcttcttcctatggaagacaattgatttggctttaacttttctgtcctttatgtccactttattaccacacaagactattgggatgttt
tcacacactctttcaagatctctatgcccgtttggtacattcttgtatgtgactcttgattgtacatcaaacctgaaaaagggccccctgggcctgggaa
ataatatccattcttctgaaaccccccaaaccttttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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